Isolation and quantification of chemical markers for standardization of dry extract of leaves Mouriri pusa Gardner (Melastomataceae)

Abstract

The use of medicinal plants for therapeutic purposes is millenary and totally diffused throughout the world. However, especially in Brazil, the informally use of plant derivatives represents a big risk to population that using these products indiscriminately without considering the possible toxicity of some components and possible drug interactions. Chemical, biological and pharmacological studies are important for validation of the traditional use of these plants. But for a medicinal plant used as raw material for the production of phytomedicine, it is necessary to search for analytical parameters that ensure the quality of these products. The registration of new products (phytomedicines) involves the determination of chemical markers as well as the development and validation of methods for the quantification of these produced in the extract and in the final product. Among the species of native flora population is traditionally used Mouriripusa Gardner (Melastomataceae) that presents considerable activity in the fight against gastritis and ulcers. Even with the chemical study ever conducted, the traditional use and scientific evidence of efficacy and safety for previous pre-clinical trials, there is still no established chemical marker for the species. This project proposes the isolation and characterization of chemicals markers (flavonoids and/or phenolic acids) and the development/validation of a methodology for quantifying these UV spectrophotometry and HPLC-UV-Vis.