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Biochemical markers associated with carbohydrate metabolism during Araucaria angustifolia zygotic and somatic embryogenesis

Grant number: 14/26888-0
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): May 01, 2015
Effective date (End): June 30, 2018
Field of knowledge:Biological Sciences - Botany - Applied Botany
Principal researcher:Eny Iochevet Segal Floh
Grantee:Bruno Viana Navarro
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):17/16667-5 - Changes in the metabolism during zygotic and somatic embryogenesis of Araucaria angustifolia, BE.EP.DD

Abstract

The plant embryogenesis is a highly complex process and organized in space and time, with a central role in the plant life cycle. The establishment and development of a new individual, the embryo by zygotic and somatic embryogenesis, has been used as a model for studies of development and differentiation in plants. Somatic embryogenesis, considered a biotechnological technique has been an alternative of clonal propagation for conservation and breeding of the species. Araucaria angustifolia is a native subtropical coniferous of Brazil that has recalcitrant seeds. Because of its economic importance, were heavily exploited over the years, currently classified as critically endangered species by IUCN. There are about twenty years, the research group of Laboratório de Biologia Celular de Plantas (BIOCEL) has used this system in plant physiological, biochemical and molecular studies, an integrative approach of zygotic and somatic embryogenesis. Different markers throughout embryogenesis has been investigated, however, carbohydrates and your metabolism, which admittedly are a key part of the processes of growth and plant development remains understudied. This project aims to investigate the role of carbohydrates and the integration of their metabolism with other markers, specifically ethylene and nitric oxide during embryogenesis zygotic and somatic A. angustifolia. For this, we used physiological, biochemical and molecular parameters such as carbohydrate profile (soluble carbohydrates, starch and cell wall), ethylene, nitric oxides, reactive oxygen species and differential expression of genes (according to the bank transcripts existing in BIOCEL). (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NAVARRO, V, BRUNO; ELBL, PAULA; DE OLIVEIRA, LEANDRO F.; PIOVEZANI, AMANDA R.; DOS SANTOS, ANDRE L. W.; DE SOUZA, DIEGO T.; DEMARCO, DIEGO; BUCKERIDGE, MARCOS S.; FLOH, ENY I. S.. Cell-to-cell trafficking patterns in cell lines of Araucariaangustifolia (Brazilian pine) with contrasting embryogenic potential. PLANT CELL TISSUE AND ORGAN CULTURE, v. 148, n. 1, . (16/17541-2, 14/50884-5, 14/26888-0)
NAVARRO, BRUNO V.; DE OLIVEIRA, LEANDRO F.; DE OLIVEIRA, LAUANA P.; ELBL, PAULA; MACEDO, AMANDA F.; BUCKERIDGE, MARCOS S.; FLOH, ENY I. S.. Starch turnover is stimulated by nitric oxide in embryogenic cultures of Araucaria angustifolia. PLANT CELL TISSUE AND ORGAN CULTURE, v. 147, n. 3, . (14/11228-5, 14/26888-0, 16/17541-2)
WENDT DOS SANTOS, ANDRE LUIS; ELBL, PAULA; NAVARRO, BRUNO VIANA; DE OLIVEIRA, LEANDRO FRANCISCO; SALVATO, FERNANDO; BALBUENA, TIAGO SANTANA; SEGAL FLOH, ENY IOCHEVET. Quantitative proteomic analysis of Araucaria angustzfolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential. JOURNAL OF PROTEOMICS, v. 130, p. 180-189, . (14/26888-0, 12/51145-6)
DE OLIVEIRA, LEANDRO FRANCISCO; NAVARRO, BRUNO VIANA; CERRUTI, GIOVANNI VICTORIO; ELBL, PAULA; MINOCHA, RAKESH; MINOCHA, SUBHASH C.; WENDT DOS SANTOS, ANDRE LUIS; SEGAL FLOH, ENY IOCHEVET. Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential. Plant and Cell Physiology, v. 59, n. 5, p. 1084-1098, . (12/22738-9, 14/26888-0, 15/21075-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
NAVARRO, Bruno Viana. Biochemical markers associated with carbohydrate metabolism during the zygotic and somatic embryogenesis of Araucaria angustifolia (Bertol.) Kuntze. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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