Currently, several studies have focused on understanding the physiology of sperm maturation in dogs, however, the lack of susceptibility of sperm epididymal the action of free radicals preclude the use of antioxidants in extender for cryopreservation of such samples. The aim of this study was to evaluate the susceptibility of canine epididymal sperm from head, body and tail to reactive oxygen species. Ten male dogs will undergo orchiectomy. After castration the epididymis will be stored at 5 ° C and the semen will be recovered within 24 hours. The samples will be obtained from the 3 regions of the epididymis: head, body and tail. The samples will be divided and incubated at 37°C for 30 minutes in 5 treatments: superoxide anion (O2-), hydrogen peroxide H2O2, hydroxyl radical (OH-), malondialdehyde and control. After incubation, the samples shall be subjected to computer analysis of sperm motility (CASA), plasma membrane integrity (eosin/nigrosine), acrosome integrity (Fast green/Rose Bengal), mitochondrial activity by oxidation of 3,3 'diaminobenzidine (DAB) and oxidative stress analysis by evaluation of thiobarbituric acid reactive species. The results will be evaluated by the SAS System for Windows (SAS, 2000) considering p <0.05.
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