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Human bone marrow mesenchymal stem cells demethylation: epigenetic regulation

Grant number: 15/02160-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): April 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Denise Carleto Andia
Grantee:Rahyza Inacio Freire de Assis
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated research grant:13/09650-8 - Epigenetic regulation in human mesenchymal stem cells, AP.JP


Although limited, studies using mesenchymal stem cells (MSCs) on regenerative therapy have brought promising results, in the last few years. Some boundaries relies on the little knowledge we have about the basic biology of these cells, mainly regarding epigenetic control landscape in the dedifferentiation and multipotentiality cellular state. Amongst several epigenetic control mechanisms, DNA methylation and demethylation mechanisms are extremely relevant in this context. Understanding this scenario may contribute to improve the biological knowledge of what is currently known as health and human development and still be used in the process of cell and tissue regeneration. Aiming to better understanding this dynamic, human bone marrow mesenchymal stem cells will be demethylated by a DNA methyltransferase (DNMT)-1 inhibitor and the role of the DNMT-1 and TETs genes (methylation and demethylation genes) and their respective enzymes will be in vitro studied. This study aims to shed some light into the epigenetic regulation and its impact on global DNA methylation and demethylation and also in some specific genes, against stimuli that can affect the molecular and cellular microenvironment, such as DNA methylation by exogenous agent. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ASSIS, RAHYZA I. F.; SCHMIDT, ARTHUR G.; RACCA, FRANCESCA; DA SILVA, RODRIGO A.; ZAMBUZZI, WILLIAM F.; SILVERIO, KARINA G.; NOCITI, FRANCISCO H.; PECORARI, VANESSA G.; WIENCH, MALGORZATA; ANDIA, DENISE C.. DNMT1 Inhibitor Restores RUNX2 Expression and Mineralization in Periodontal Ligament Cells. DNA AND CELL BIOLOGY, v. 40, n. 5, . (13/09650-8, 15/02160-0, 17/07944-5)
ASSIS, RAHYZA I. F.; WIENCH, MALGORZATA; SILVERIO, KARINA G.; DA SILVA, RODRIGO A.; FELTRAN, GEORGIA DA SILVA; SALLUM, ENILSON A.; CASATI, MARCIO Z.; NOCITI, JR., FRANCISCO H.; ANDIA, DENISE C.. RG108 increases NANOG and OCT4 in bone marrow-derived mesenchymal cells through global changes in DNA modifications and epigenetic activation. PLoS One, v. 13, n. 12, . (13/09650-8, 15/02160-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ASSIS, Rahyza Inacio Freire de. Mesenchymal stem cell epigenetic modulation after DNA methyltransferase inhibitor RG108 treatment. 2017. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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