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Toxicogenomic and biochemical approaches to toxicity assessment of natural and synthetic dyes to zebrafish

Grant number: 14/27009-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2016
Field of knowledge:Biological Sciences - Pharmacology - Toxicology
Principal researcher:Danielle Palma de Oliveira
Grantee:Flavia Renata Abe
Supervisor abroad: Amadeu Mortágua Velho da Maia Soares
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: Universidade de Aveiro (UA), Portugal  
Associated to the scholarship:13/14397-0 - Ecogenotoxicological evaluation of natural dye extracted from microorganism, BP.DR


Organic compounds present in natural resources have been used in several experimental conditions aimed to develop safe human health dyes as alternative to dyeing industries, replacing synthetic dyes, since these are precursors of mutagenic compounds. Moreover, the use of natural dyes can add value to the final product. Many phenolic compounds extracted from microorganisms are potential natural dyes and have antioxidant activity due to the number of conjugated double bonds in the molecule. Nonetheless, in addition to a safe human health dye, an eco-friendly dye is required to be used by industries, once the international scientific community is concerned about environmental health. Thus, toxicity tests to non-target organisms have been required to evaluate adverse effects to the ecosystem. Zebrafish embryos are becoming an important tool as alternative model in ecotoxicology, due to the easy maintenance and breeding. Zebrafish embryos tests allow to analyze morphological alterations, survival and gene expression in a unique assay. Thus, the present work aims to study the natural phenolic dye erythrostominone and its photodegraded extracted from microorganism, and the synthetic dye Basic Red 51, in order to investigate early compound-induced gene expression changes and biochemical alterations potentially leading to morphological effects to zebrafish embryos (D. rerio), and to compare the morphology, transcriptomics and biochemical data among natural and synthetic dyes. The toxicogenomics potential of the dyes will be performed in zebrafish embryos by microarray analysis to verify gene expression, also biochemical changes by biomarkers analysis. Finally, we will compare the morphological, transcriptomic and biochemical data of natural and synthetic dyes, and verify sub-morphological effects to improve the predictability of the test. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ABE, FLAVIA R.; GRAVATO, CARLOS; SOARES, AMADEU M. V. M.; DE OLIVEIRA, DANIELLE P.. Biochemical approaches to assess oxidative stress induced by exposure to natural and synthetic dyes in early life stages in zebrafish. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 80, n. 23-24, p. 1259-1268, . (13/14397-0, 14/27009-0, 13/01509-4)
ABE, FLAVIA R.; SOARES, AMADEU M. V. M.; DE OLIVEIRA, DANIELLE P.; GRAVATO, CARLOS. Toxicity of dyes to zebrafish at the biochemical level: Cellular energy allocation and neurotoxicity. Environmental Pollution, v. 235, p. 255-262, . (13/14397-0, 14/27009-0, 13/01509-4)
ABE, FLAVIA R.; MACHADO, ANA L.; SOARES, AMADEU M. V. M.; DE OLIVEIRA, DANIELLE P.; PESTANA, JOAO L. T.. Life history and behavior effects of synthetic and natural dyes on Daphnia magna. Chemosphere, v. 236, . (13/14397-0, 14/27009-0, 13/01509-4)
ABE, FLAVIA R.; MENDONCA, JACQUELINE N.; MORAES, LUIZ A. B.; DE OLIVEIRA, GISELE A. R.; GRAVATO, CARLOS; SOARES, AMADEU M. V. M.; DE OLIVEIRA, DANIELLE P.. Toxicological and behavioral responses as a tool to assess the effects of natural and synthetic dyes on zebrafish early life. Chemosphere, v. 178, p. 282-290, . (13/14397-0, 14/27009-0)

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