Effect of cranberry and proanthocyanidin in inhibiting wear and degradation of the organic matrix of demineralised dentine

Abstract

The literature recognizes that one of the most effective strategies in minimizing dentin structural loss exposed to erosive agents is maintaining the organic matrix, since its acts as a diffusion barrier to acid. However, the collagen tends to be degraded and has been more frequent to search for agents that inhibit this process. There are indications that the cranberry and proanthocyanidin (its active ingredient) have the potential to inhibit Matrix Metalloproteinases (MMPs) that degrade the dentin collagen, but these agents have not been properly evaluated. The objective of this study is to evaluate the role of cranberry extract and its main active agent (proanthocyanidin) applied as a topical gel in the inhibition of the degradation of the organic matrix of demineralized dentin and thus minimizing the dentin wear subjected to erosion. For this study, dentin specimens (4 mm thick) in 5 ml of demineralized citric acid 0.87 M, pH 2.3, for 36 h at 4°C are used. Then, the specimens will be divided and submitted to the protocol of the 5 experimental groups of this study: G1 specimens treated with proanthocyanidin gel 10% (test 1), G2 specimens treated with cranberry gel 10% (test 2) G3 specimens treated with chlorhexidine gel 0.012% (positive control 1), G4 specimens treated with 1.23% NaF gel (positive control 2) and G5 specimens treated with placebo gel (negative control 1). Each group will be demineralized collagen degradation by the action of collagenase from Clostridium histolyticum (100 U / ml), added with 5 ml of artificial saliva containing EDTA- free protease inhibitor for 5 days at 37°C. The samples are 1000 X concentrated HCl and hydrolyzed with 6 mol / ml at 98°C for 24 hours. Will again lyophilized and resuspended in water (600 ul) and then the levels of hydroxyproline is performed (method 1. Assessment of loss of dentin matrix will be made by profilometry (method 2). The data will be submitted to ANOVA followed by Tukey test if these parameters comply normal distribution and homogeneity of variances. If it does not, the nonparametric version of this test (Kruskal-Wallis) will be used. (AU)