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Study of the action of bovine papillomavirus (BPV) in epithelial-mesenchymal transition

Grant number: 14/20617-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2015
Effective date (End): December 31, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Rita de Cassia Stocco
Grantee:Rodrigo Pinheiro Araldi
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Bovine papillomavirus (BPV) is the etiologic agent of bovine papillomatosis, infectious disease characterized by the presence of papillomas that regress spontaneously but can persist and progress to malignancy. The virus is considered the best model in understanding the oncogenic process of HPV. Studies suggest that papillomavirus induces epithelial-mesenchymal transition (EMT), which is characterized by morphological and transcriptional changes, conferring loss of cell adhesion and gain of motility. Although the association between papillomas and cancer has been known for more than 60 years, the mechanisms of malignant transformation, invasion and metastasis remain unknown. This work aims to evaluate the role of BPV in EMT in both cell culture and tissue samples (cutaneous papilloma tissue and esophageal carcinoma). Cutaneous papilloma, carcinoma of the esophagus and normal skin (control) cells will be cultured until passage six, evaluating the cell cycle by FACScan and the levels of clastogenicity by comet assay. The molecular diagnosis of BPV will be performed by PCR and sequencing. The expression of different markers of EMT, as well as the production of reactive oxygen species will be evaluated by FACScan and immunofluorescence. The migration and invasion assays will be used to assess cell motility. Samples from the same tissue that originated cell lines will be subjected to histopathological and immunohistochemical analysis using the same markers for EMT evaluated in vitro. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ARALDI, RODRIGO PINHEIRO; SANT'ANA, THALITA ARAUJO; MODOLO, DIEGO GRANDO; DE MELO, THATIANA CORREA; SPADACCI-MORENA, DIVA DENELLE; STOCCO, RITA DE CASSIA; CERUTTI, JANETE MARIA; DE SOUZA, EDISLANE BARREIROS. The human papillomavirus (HPV)-related cancer biology: An overview. BIOMEDICINE & PHARMACOTHERAPY, v. 106, p. 1537-1556, . (17/14948-7, 14/20617-5)
ARALDI, RODRIGO PINHEIRO; MAZZUCHELLI-DE-SOUZA, JACQUELINE; MODOLO, DIEGO GRANDO; DE SOUZA, EDISLANE BARREIROS; DE MELO, THATIANA CORREA; SPADACCI-MORENA, DIVA DENELLE; MAGNELLI, ROBERTA FIUSA; CALDAS ROCHA DE CARVALHO, MARCIO AUGUSTO; DE SA JUNIOR, PAULO LUIS; DE CARVALHO, RODRIGO FRANCO; et al. Mutagenic Potential of Bos taurus Papillomavirus Type 1 E6 Recombinant Protein: First Description. BIOMED RESEARCH INTERNATIONAL, . (14/20617-5)
ARALDI, RODRIGO PINHEIRO; DE MELO, THATIANA CORREA; MENDES, THAIS BIUDE; DE SA JUNIOR, PAULO LUIZ; NAKANO NOZIMA, BRUNO HEIDI; ITO, ELIANA TIEMI; DE CARVALHO, RODRIGO FRANCO; DE SOUZA, EDISLANE BARREIROS; STOCCO, RITA DE CASSIA. Using the comet and micronucleus assays for genotoxicity studies: A review. BIOMEDICINE & PHARMACOTHERAPY, v. 72, p. 74-82, . (14/20617-5)
ARALDI, RODRIGO PINHEIRO; DOS SANTOS, MARISTELA OLIVEIRA; BARBON, FABIANE FARIA; MANJERONA, BRUNA APARECIDA; MEIRELLES, BRUNO ROSA; NETO, PEDRO DE OLIVA; DA SILVA JUNIOR, PEDRO ISMAEL; DOS SANTOS, LUCINEIA; CHERICI CAMARGO, ISABEL CRISTINA; DE SOUZA, EDISLANE BARREIROS. Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes. BIOMEDICINE & PHARMACOTHERAPY, v. 98, p. 873-885, . (14/20617-5)
ARALDI, RODRIGO PINHEIRO; MODOLO, DIEGO GRANDO; DE SA JUNIOR, PAULO LUIZ; CONSONNI, SILVIO ROBERTO; DE CARVALHO, RODRIGO FRANCO; ROPERTO, FRANCO PEPPINO; BECAK, WILLY; STOCCO, RITA DE CASSIA. Genetics and metabolic deregulation following cancer initiation: A world to explore. BIOMEDICINE & PHARMACOTHERAPY, v. 82, p. 449-458, . (14/20617-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ARALDI, Rodrigo Pinheiro. Cell lines derived from BPV-infected neoplasms primary cultures as model to study epitelial-mesenchymal transition.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.