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DNA methylation in HeLa cells after treatment with valproic acid

Grant number: 14/23842-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Maria Luiza Silveira Mello
Grantee:Giovana Maria Breda Veronezi
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:10/50015-6 - Chromatin structure and organization with aging and diabetes compared to induced changes in epigenetic markers, AP.TEM


Valproic Acid (VPA) is a well known Histone Deacetylase (HDAC) inhibitor. Currently, in addition to HDAC inhibition, DNA demethylation is being considered as part of the VPA action on certain cell types. In the present work, the process of DNA demethylation following VPA treatment will be studied in detail in HeLa cells, in which chromatin remodeling attributed to HDAC inhibition has been reported by our group. Previously obtained image analysis data will be expanded to establish patterns of chromatin remodeling as affected by DNA demethylation. Immunocytochemical assays and flow cytometry will be carried out to quantify the DNA methylation abundance. In addition, the Fourier Transform-Infrared (FT-IR) spectrum will be obtained by microspectroscopy to detect spectral changes that may occur as a function of vibrational changes in the DNA molecule due to DNA demethylation. Because the VPA action may be associated to the recently described enzymes named Ten-Eleven Translocation Methylcytosine Dioxygenases (TET), the levels of gene expression and the abundance of these enzymes as well as the levels of DNA hydroxymethylation will also be investigated. It is expected that a better knowledge on the dynamic action of VPA on DNA methylation/demethylation mechanisms could be revealed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VERONEZI, GIOVANA M. B.; FELISBINO, MARINA BARRETO; GATTI, MARIA SILVIA V.; MELLO, MARIA LUIZA S.; VIDAL, BENEDICTO DE CAMPOS. DNA Methylation Changes in Valproic Acid-Treated HeLa Cells as Assessed by Image Analysis, Immunofluorescence and Vibrational Microspectroscopy. PLoS One, v. 12, n. 1, . (14/23842-0)
ROCHA, MARINA AMORIM; BREDA VERONEZI, GIOVANA MARIA; FELISBINO, MARINA BARRETO; VICCARI GATTI, MARIA SILVIA; TAMASHIRO, WIRLA M. S. C.; SILVEIRA MELLO, MARIA LUIZA. Sodium valproate and 5-aza-2 `-deoxycytidine differentially modulate DNA demethylation in G1 phase-arrested and proliferative HeLa cells. SCIENTIFIC REPORTS, v. 9, . (14/23842-0, 15/10356-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VERONEZI, Giovana Maria Breda. The DNA methylation in HeLa cells after valproic acid treatment. 2017. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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