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Multimodal array of enzymatic biosensors: potentiometric differential mode, optical and conductometric.

Grant number: 14/24559-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2015
Effective date (End): March 31, 2019
Field of knowledge:Engineering - Electrical Engineering
Principal Investigator:Marcelo Mulato
Grantee:Hugo José Nogueira Pedroza Dias Mello
Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:12/22820-7 - The sensitive label free detection of disease markers using electrochemical immittance, AP.TEM
Associated scholarship(s):16/23305-0 - Study of polymers structure-property relationship and its applications to organic electronics sensor devices, BE.EP.DR


In this work we intend to develop and refine chemical biosensors with high selectivity to biological elements. Three chemical pH sensors, with sensitive films based on nanostructured polyaniline (PANI) and differential mode potentiometric, optical and conductometric transduction, will be studied to serve as the basis of enzymatic biosensors. Subsequently, the multimodal array of enzymatic biosensors (MMBE) will be built. The differential potentiometric sensor EGFET (Extended Gate Field Effect Transistor) requires the development of materials for the principal and contrast film REFET, responsible for the contrast signal that ensures adaptation of the system to variation of environmental parameters. For enzymatic biosensor used to detect glucose and urea, make a nanostructured functionalized PANI film by immobilization of catalyst enzymes is important. The functionalized films will be applied in biosensors with transduction potentiometric in differential mode, optical and conductometric. Using these biosensors developed in conjunction with chemical sensors, the MMBE for the simultaneous detection of the analyte with the system adapted to environmental changes will be build. With the device MMBE developed, tested and validated, it will be possible to propose a miniaturized prototype by microfabrication techniques from the electronics industry. These sensors have great significance and wide application in medicine and biology.

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEDROZA DIAS MELLO, HUGO JOSE NOGUEIRA; MULATO, MARCELO. PANI/PPY blend thin films electrodeposited for use in EGFET sensors. Journal of Applied Polymer Science, v. 135, n. 33, . (14/24559-0, 14/09562-4)
PEDROZA DIAS MELLO, HUGO JOSE NOGUEIRA; MULATO, MARCELO. Well-established materials in microelectronic devices systems for differential-mode extended-gate field effect transistor chemical sensors. MICROELECTRONIC ENGINEERING, v. 160, p. 73-80, . (14/09562-4, 14/24559-0)
PEDROZA DIAS MELLO, HUGO JOSE NOGUEIRA; BACHOUR JUNIOR, BASSAM; MULATO, MARCELO. Polyaniline-based field effect transistor for DNA/RNA biomarker sensing: Comparison to electrochemical impedance and inorganic layer. SENSORS AND ACTUATORS A-PHYSICAL, v. 318, . (17/24201-6, 14/24559-0)
MADEIRA, GUSTAVO DANIEL M.; DIAS MELLO, HUGO JOSE N. P.; FALEIROS, MURILO C.; MULATO, MARCELO. Model improvement for super-Nernstian pH sensors: the effect of surface hydration. Journal of Materials Science, v. 56, n. 3, p. 2738-2747, . (18/24395-8, 14/24559-0, 18/22223-5, 17/24201-6)
PEDROZA DIAS MELLO, HUGO JOSE NOGUEIRA; MULATO, MARCELO. Effect of aniline monomer concentration on PANI electropolymerization process and its influence for applications in chemical sensors. Synthetic Metals, v. 239, p. 66-70, . (14/09562-4, 14/24559-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MELLO, Hugo José Nogueira Pedroza Dias. Solid-state biosensors and field-effect transistor devices based on organic semiconductors. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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