Multimodal array of enzymatic biosensors: potentiometric differential mode, optical and conductometric.

Abstract

In this work we intend to develop and refine chemical biosensors with high selectivity to biological elements. Three chemical pH sensors, with sensitive films based on nanostructured polyaniline (PANI) and differential mode potentiometric, optical and conductometric transduction, will be studied to serve as the basis of enzymatic biosensors. Subsequently, the multimodal array of enzymatic biosensors (MMBE) will be built. The differential potentiometric sensor EGFET (Extended Gate Field Effect Transistor) requires the development of materials for the principal and contrast film REFET, responsible for the contrast signal that ensures adaptation of the system to variation of environmental parameters. For enzymatic biosensor used to detect glucose and urea, make a nanostructured functionalized PANI film by immobilization of catalyst enzymes is important. The functionalized films will be applied in biosensors with transduction potentiometric in differential mode, optical and conductometric. Using these biosensors developed in conjunction with chemical sensors, the MMBE for the simultaneous detection of the analyte with the system adapted to environmental changes will be build. With the device MMBE developed, tested and validated, it will be possible to propose a miniaturized prototype by microfabrication techniques from the electronics industry. These sensors have great significance and wide application in medicine and biology.