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VEGF- and CXCL12-LOADED injectable hydrogel for mesenchymal stem cell delivery in a traumatic brain injury model

Grant number: 14/23797-4
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 15, 2015
Effective date (End): October 13, 2015
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Marimélia Aparecida Porcionatto
Grantee:Laura Nicoleti Zamproni
Supervisor: Anne des Rieux
Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Research place: Universitè Catolique de Louvain (UCL), Belgium  


Brain injuries are potentially disabling, generate a high social cost and no neuroprotective/neuroregenerative strategies are clinically available at the moment. Stem cell therapy seems to be a promising strategy for brain repair. Two main strategies have been proposed: 1) exogenous stem cell transplantation, and 2) subventricular zone (SVZ) derived neural stem cell mobilization. The difficulties that are encountered depend on the strategy. Transplanted exogenous stem cells show poor engraftment at the lesion site and the brain-blood barrier is an obstacle for cell and drug delivery. On the other hand, endogenous neural stem cell mobilization relies on physiological neurogenesis that is not sufficient to induce clinical and functional recovery. Thus, approaches to improve the activation of the physiologic neurogenesis may lead to better outcome following injury. Scaffolds that can be used to deliver stem cells as well as fix them at the injury site are an alternative for cell delivery. Recently, authors have described attempts to deliver stem cells combined with chemoattractants and growth factors to enhance neuroprotection and neuroblast influx from the SVZ.In this project, we propose to use scaffolds to deliver a combination of biomolecules and stem cells to the brain. Hydrogels containing the proangiogenic factor VEGF, the chemokine CXCL12 and/or stem cells will be layered on top of organotypic sections of mouse brain submitted to a traumatic injury. Neuroblast influx to the lesion and angiogenesis will be evaluated by immuno-histochemistry. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZAMPRONI, LAURA N.; TEIXEIRA, DANIELA; ALLIEGRO, AMANDA A.; MAUGERI, IEDA L.; DES RIEUX, ANNE; PORCIONATTO, MARIMELIA A.. Decreased viability and neurite length in neural cells treated with chitosan-dextran sulfate nanocomplexes. NeuroToxicology, v. 76, p. 33-43, . (12/00652-5, 14/23797-4)
ZAMPRONI, LAURA N.; MUNDIM, MAYARA T. V. V.; PORCIONATTO, MARIMELIA A.. Neurorepair and Regeneration of the Brain: A Decade of Bioscaffolds and Engineered Microtissue. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 09, p. 16-pg., . (14/00927-0, 14/23797-4, 18/12605-8, 13/16533-8, 12/06810-1)
ZAMPRONI, LAURA N.; MUNDIM, MAYARA T. V. V.; PORCIONATTO, MARIMELIA A.. eurorepair and Regeneration of the Brain: A Decade of Bioscaffolds and Engineered Microtissu. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 09, . (18/12605-8, 12/06810-1, 14/00927-0, 14/23797-4, 13/16533-8)

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