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Two-dimensional Ultra-Efficiency Liquid Chromatography with Detection by Tandem Mass Spectrometry (2D-UPLC-MS/MS) for determination of drugs in plasma samples from schizophrenic patients

Grant number: 14/22140-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): February 01, 2015
Effective date (End): April 30, 2018
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Maria Eugênia Queiroz Nassur
Grantee:Vinicius Ricardo Acquaro Junior
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):16/16180-6 - Determination of AEA and 2-AG in plasma samples by Bio-SPME-Nano-ESI, BE.EP.DR


Schizophrenia is a neuropsychiatric disorder that affects approximately 1% of world population, characterized, among other factors by the loss or impairment of mental ability, hallucinations, delusions, with loss of contact with reality, and can result in a chronic social dysfunction. Apart from antipsychotics, most patients also make concomitant use of other classes of drugs such as antidepressants, anticonvulsants and anxiolytics, in order to reduce the symptoms associated with this disease. Consequently, the analysis of these drugs in plasma samples of schizophrenic patients is important to adjust doses, minimize adverse effects, and verify patient compliance to therapy. In recent years, ultra-efficiency liquid chromatography coupled to mass spectrometry, tandem system (UPLC-MS/MS), has become the analytical technique of reference for bioanalysis, and developing selective sorbents phases such as the materials with restricted access (RAM - restricted acess media), has allowed the direct introduction of biological fluids in chromatographic systems, reducing the time of analysis. The new generation of superficially porous columns, comprised of a solid core coated with a thin layer of porous sílica and particles with diameters sub-3¼m, compared to completely porous particles with diameters sub-2¼m, showed chromatographic separation with equal or greater efficiency, but with significant reduction in pressure of the chromatographic system. In this project the two-dimensional chromatographic systems 2D-UPLC-MS/MS with column RAM in the first dimension and reversed-phase column in the second dimension will be used to the development of an analytical method for the simultaneous determination of drugs (antipsychotics, antidepressants, anticonvulsants and anxiolytics) in plasma samples from schizophrenic patients. Different columns in reverse phase, totally porous, and porous surface with different particle sizes are evaluated in the first dimension of the system 2D-UPLC for the separation of drugs.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ACQUARO JUNIOR, VINICIUS RICARDO; LANCAS, FERNANDO MAURO; COSTA QUEIROZ, MARIA EUGENIA. Evaluation of superficially porous and fully porous columns for analysis of drugs in plasma samples by UHPLC-MS/MS. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1048, p. 1-9, MAR 24 2017. Web of Science Citations: 5.
ACQUARO JUNIOR, VINICIUS RICARDO; DOMINGUES, DIEGO SOARES; COSTA QUEIROZ, MARIA EUGENIA. Column switching UHPLC-MS/MS with restricted access material for the determination of CNS drugs in plasma samples. BIOANALYSIS, v. 9, n. 6, p. 555-568, MAR 2017. Web of Science Citations: 4.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
JUNIOR, Vinicius Ricardo Acquaro. Development of different LC-MS/MS methods for the determination of drugs and endocannabinoids in plasma samples. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Ribeirão Preto.

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