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Analysis of bonding interface between self-adhesive resin cement to in vitro eroded dentin

Grant number: 14/11734-8
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Paulo Henrique dos Santos
Grantee:Mariana Dias Moda
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The purpose of this study will be evaluate the bonding interface between self-adhesive resin cement to in vitro eroded dentin. Sixty-four third molars will be used and divided into two groups: group 1 (standard dentin) and group 2 (eroded dentin). The in-vitro erosion will be created following a demineralization protocol, in which the specimens of group 2 will be immersed in HCl-pepsin solutions during 5 minutes per cycle, and in trypsin solution, 10 minutes per cycle, totaling 6 immersions per day on both solutions; and manually brushed, 2 times per day during 6 days. Both groups will be submitted to four dentin surface treatments: control group (without any treatment), 25%polyacrylic acid, 2% chlorhexidine and 0,1M EDTA pH 7.4 (n=8). TPH resin-based composite blocks will be bonded with RelyX U200 self-adhesive resin cement on the pretreated dentin surfaces. The samples will be submitted to microtensile bonding test to evaluate the bonding strength between self-adhesive resin cement to dentine, 24 hours and 1 year after the bonding procedure. The fractured samples will be evaluated in Scanning Electron Microscopy (SEM), to classify the fracture pattern of these interfaces. Finally, two specimens of each group will be evaluating by laser confocal microscopy, with the purpose to analyze qualitatively the dentin/cement interface. Data of microtensile bonding strength and dentin/cement interface quality will be submitted to statistical tests. (p=0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MODA, MARIANA DIAS; FAGUNDES, TICIANE CESTARI; FRAGA BRISO, ANDRE LUIZ; DOS SANTOS, PAULO HENRIQUE. Analysis of the bond interface between self-adhesive resin cement to eroded dentin in vitro. PLoS One, v. 13, n. 11 NOV 26 2018. Web of Science Citations: 1.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MODA, Mariana Dias. Analysis of bonding interface between self-adhesive resin cement to in vitro eroded dentin. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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