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Optimization of the solvent mixture for extraction of phenolic compounds with antioxidant and antiproliferative activities from Eugenia punicifolia (Kunth) DC leaves by applying the simplex-centroid design

Grant number: 14/19504-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2014
Effective date (End): July 31, 2015
Field of knowledge:Engineering - Chemical Engineering - Chemical Technology
Principal Investigator:Catarina dos Santos
Grantee:Andressa Lie Mizobuchi
Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil


The phenolic compounds derived from plants are widely used for therapeutic application. The ones that possess antioxidant activity are highlighted for reducing damage related to aging and the genesis of diseases. In relation to Eugenia punicifolia (Myrtaceae), it is known its anti-inflammatory action, the effect on the nicotinic-cholinergic neurotransmission and the use for the treatment of diabetes. For a better extraction and to potentiate the compounds related to these biological activity it is common to use statistical design of solvent mixtures. For this reason, in this project, it will be done the optimization of a mixture of solvents methanol, ethanol and water to obtain leaf extracts of E. punicifolia rich in phenolic and/or flavonoid compounds with a higher antioxidant and antiproliferative activity possible. The planning of the solvent mixtures will be made according to the simplex-centroid design. The extracts will be analyzed for total phenols with Folin-Ciocalteau reagent, total flavonoids with aluminum chloride and antioxidant activity by the method of radical kidnapping DPPH (2,2-diphenyl-1-picrylhydrazyl). Toxicity assays and cell proliferation will be carried out by using a colorimetric assay in the presence of MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide against three cell types: MCF-7 (human breast cancer), HL60 (human promyelocytic line ) and human peripheral blood monomuclear leukocytes.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, CATARINA; MIZOBUCCHI, ANDRESSA LIE; ESCARAMBONI, BRUNA; LOPES, BRUNO PEREIRA; FIGUEIREDO ANGOLINI, CELIO FERNANDO; EBERLIN, MARCOS NOGUEIRA; DE TOLEDO, KARINA ALVES; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO. Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities. BMC CHEMISTRY, v. 14, n. 1, . (14/19504-1, 17/15610-0)

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