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Role of the SepA serine protease in the cytotoxic and proinflammatory activities induced by enteroaggregative Escherichia coli

Grant number: 14/18286-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2014
Effective date (End): November 14, 2018
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Waldir Pereira Elias Junior
Grantee:Fernanda Batista de Andrade
Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated scholarship(s):17/25248-6 - Intracellular trafficking of SepA, BE.EP.DR


Enteroaggregative Escherichia coli (EAEC) is an emerging enteropathogen among the bacterial agents of diarrhea worldwide. Nowadays a strong association has been reported between EAEC and acute and persistent diarrhea in children in both developing and developed countries. In 2011 a large outbreak of diarrhea, which affected several countries in Europe, was caused by a strain of Shiga toxin-producing E. coli. Sequencing this strain showed that it was an EAEC that acquired a phage encoding Stx2, a characteristic of Shiga toxin-producing E. coli (STEC). This strain has several virulence factors including the SPATE proteins (Serine Protease Autotransporters of Enterobacteriaceae), which are secreted to the extracellular milieu by the type V secretion system, also known as autotransporter system. The SPATEs are distributed among the Enterobacteriaceae family and are defined as important virulence factors in many pathogens. SepA is a SPATE, originally described in Shigella flexneri 2a and later in EAEC, which belong to the class 2 SPATE that include autotransporters with immunomodulatory activities. There are reports describing SepA causing destruction of epithelial tissue; however, without cytopathic effect on HEp-2 cells. Also, its cellular target is still unknown. EAEC strains that present SepA protein are associated with cases of diarrhea, but its role in the pathogenicity of EAEC remains unknown. Thus, this study aims to determine the function of this autotransporter toxin in some pathogenic mechanisms of EAEC. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANDRADE, FERNANDA B.; ABREU, AFONSO G.; NUNES, KAMILA O.; GOMES, TANIA A. T.; PIAZZA, ROXANE M. F.; ELIAS, WALDIR P.. Distribution of serine protease autotransporters of Enterobacteriaceae in typical and atypical enteroaggregative Escherichia coli. INFECTION GENETICS AND EVOLUTION, v. 50, p. 83-86, . (14/18286-0, 15/15135-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ANDRADE, Fernanda Batista de. Interaction of the serine protease SepA of enteroaggregative Escherichia coli with epithelial cells in vitro.. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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