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Cryopreservation of spermatozoa from Rhesus macaques (Macaca mulatta) and assessment of frozen-thawed semen quality by IVF

Grant number: 14/15847-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): February 01, 2015
Effective date (End): January 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Mario Binelli
Grantee:Fernanda Maria de Carvalho
Supervisor abroad: Jon D. Hennebold
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Oregon National Primate Research Center (ONPRC), United States  
Associated to the scholarship:12/24709-6 - EVALUATION OF DIFFERENT CRYOPRESERVATION METHODS AND THE EFFECT OF TREATMENT WITH ANTI-OXIDANT AGENTS IN THE POST-THAW SEMINAL CHARACTERISTICS OF HOWLER MONKEYS (Alouatta caraya), BP.DR

Abstract

There is increasing need of nonhuman primates (NHPs) for biomedical research, which generates the need of efficient methods to ensure adequate supply. The use of ARTs have overcome many of the problems encountered. The rhesus monkey (Macaca mulatta) is one of the most commonly used NHP in research. Although, there have been many advances in the application of ARTs in this species, cryopreservation of sperm is still a major limitation. The objectives of this project are to test and compare different protocols for cryopreservation of sperm from rhesus monkeys and evaluate its efficacy by assessment of post-thaw survival and in vitro fertilization rates. Semen will be collected from five adult males by penile electroejaculation and frozen by two different methods (1) a slow freezing method and (2) a vitrification method. Oocytes will be collected from five adult females by laparoscopic follicular aspiration. Frozen-thawed sperm will be used for in vitro fertilization. Embryos will be examined daily until they reach the blastocyst stage. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CARVALHO, FERNANDA M.; RAMSEY, CATHY; HANNA, CAROL B.; DO VALLE, RODRIGO DEL R.; NICHI, MARCILIO; BINELLI, MARIO; GUIMARAES, V, MARCELO A. B.; HENNEBOLD, JON D.. Cryopreservation and Preparation of Thawed Spermatozoa from Rhesus Macaques (Macaca mulatta) for In Vitro Fertilization. Journal of the American Association for Laboratory Animal Science, v. 60, n. 4, p. 396-406, . (14/15847-1)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.