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Effects of experimental periodontal disease induced by Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in the fetal-placental unit and systemic inflammatory markers

Grant number: 14/10549-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): September 01, 2014
Effective date (End): July 31, 2016
Field of knowledge:Health Sciences - Dentistry - Periodontology
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Marcia Pinto Alves Mayer
Grantee:Marcela Yang Hui Zi
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Epidemiological studies had shown that periodontitis were associated with low weight and premature birth. However, little is known about the mechanisms involved in this association. The complex microbiota associated with periodontitis includes Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, which are considered among the main periodontopathogens. Although these organisms are able to induce distinct host responses, their potential in inducing systemic alterations has not been compared. In a pilot study, we observed that the oral infection with P. gingivalis in a mouse experimental model resulted in alveolar bone loss, disseminated infection, alteration in cytokines profile and reduction in fetal weight. However, due to the low number of studied animals, these conclusions were not definitive. This study aims to test the hypothesis that differences in the oral microbiota may have lead to differences in the potential to induce gestational alterations. The effect of experimental periodontal diseases induced by P. gingivalis strain HW24 D-1 and A. actinomycetemcomitans clone JP2 on the gestational pattern and inflammatory markers will be evaluated in C57BL6 female mice. The alveolar bone loss will be determined by microtomography (microCT). The amount of colonizing pathogens will be determined by qPCR in oral biofilm, gingival tissue, blood, serum, liver, linfonodes, gut, feces, fetus, placenta and amniotic liquid samples. The effect of the infection will be evaluated by determining the maternal weight gain, weight and number of fetus and fetal resorption. The level of the inflammatory markers IL1-², IL-2, IL-6, IL-5, IL-8, IL-10, IL-17, IL-18, TNF-±, GM-CSF, IFN-³, MIP-1, MCP-1, PGE-2 and RANKL will be determined by ELISA in serum, placenta and gingival tissue. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HUI ZI, MARCELAYANG; LONGO, PRISCILA LARCHER; BUENO-SILVA, BRUNO; ALVES MAYER, MARCIA PINTO. Mechanisms involved in the association between periodontitis and complications in pregnancy. FRONTIERS IN PUBLIC HEALTH, v. 2, p. 13-pg., . (14/10549-2, 11/10057-4, 12/01500-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ZI, Marcela Yang Hui. Effects of experimental infection with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in mice pregnancy pattern and inflammatory markers.. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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