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Periodontal tissues response to tricalcium silicate cement associated to zirconium oxide or niobium oxide in rat molars

Grant number: 14/14688-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2014
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Dentistry - Endodontics
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Paulo Sergio Cerri
Grantee:Derik Damasceno Barbosa
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Calcium silicate-based cements, such as MTA, are indicated for sealing endodontics perforations due to its adequate physicochemical and biological properties. However, it has been demonstrated that the bismuth oxide (Bi2O3), radiopacifying agent, exhibits unfavorable characteristics. Therefore, other materials have been studied to replace the bismuth oxide, such as zirconium oxide (ZrO2) and niobium oxide (Nb2O5). The aim of this study is to evaluate the tissue response promoted by the tricalcium silicate (CS) associated to ZrO2 or Nb2O5, comparing to MTA-Angelus, in furcal perforations of rat molars. Eighty rats will be used and distributed into 4 groups: CS + ZrO2, CS + Nb2O5, MTA and Sham. In each animal, the pulp chamber floors of maxillary right first molar will be perforated and sealed with one of the materials (CS + ZrO2, CS + Nb2O5 or MTA); the access cavity will be restored with light-cured glass ionomer cement. In the Sham group, the furcal perforations will be not sealed; the left first molars, without any treatment, will be also used as control group. After 7, 15, 30 and 60 days the maxillae will be removed, fixed in formaldehyde and, after decalcification 4,3% EDTA solution, the fragments will be dehydrated and embedded in paraffin. Sections will be stained with haematoxylin and eosin (H&E) and Masson's trichrome. In the H&E-stained sections, the number of inflammatory cells/mm2 and the width of the periodontal space will be obtained. Other sections will be used for immunohistochemical detection of interleukin-6 and osterix. Some sections will be submitted to tartrate-resistant acid phosphatase (TRAP) reaction to quantify the number of TRAP-positive osteoclasts in the bone surface. Data will be submitted to the statistical analyses using analysis of variance and Tukey test (pd0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARBOSA, D. D.; DELFINO, M. M.; GUERREIRO-TANOMARU, J. M.; TANOMARU-FILHO, M.; SASSO-CERRI, E.; SILVA, G. F.; CERRI, P. S.. Histomorphometric and immunohistochemical study shows that tricalcium silicate cement associated with zirconium oxide or niobium oxide is a promising material in the periodontal tissue repair of rat molars with perforated pulp chamber floors. International Endodontic Journal, v. 54, n. 5, . (14/14688-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BARBOSA, Derik Damasceno. Periodontal tissues response to tricalcium silicate cement associated to zirconium oxide or niobium oxide used in the sealing of furcation perforations in rat molars. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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