Scholarship 14/13143-7 - Morfologia animal, Carrapatos - BV FAPESP
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Study morphophysiological salivary gland, singânglio, body of ovarian gene and engorged females of R. sanguineus (Latreille, 1806) (Acari: Ixodidae) treated with different concentrations thymol

Grant number: 14/13143-7
Support Opportunities:Scholarships in Brazil - Doctorate
Start date until: September 01, 2014
End date until: August 31, 2018
Field of knowledge:Biological Sciences - Parasitology
Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Maria Izabel Souza Camargo
Grantee:Renata da Silva Matos
Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

Thymol is a monoterpene action with ticks previously proven by several authors for different tick species and at different stages of life. However, little is known about the physical and physiological changes caused by this substance when used in controlling these arthropods. Thus, the aim of this project is to analyze what are the physical and physiological changes that occur in the salivary glands, singânglio, Gené body and ovaries of female Rhipcephalus sanguineus with six days of feeding, when treated with thymol at different concentrations. To do so, tick fasting will be fed on artificial infestations in rabbits as proposed by Bechara et al. (1995). After six days of feeding they will be removed from hosts and submitted to immersion test by Drummond et al. (1973) thymol in solutions at different concentrations, in Matos et al. (2013 ). Soon after the ticks will be kept for five days in a climatic chamber and after this period they will be anesthetized by heat shock and dissected for removal of various organs which, in sequence, will be subjected to histological techniques and embedded in resin and stained with hematoxylin and eosin ( HE ) and will be subjected to histochemical techniques to detect calcium and polysaccharides. Moreover, specifically ovaries and salivary glands are undergoing technique for detection of acid phosphatase activity, and body Gené will also be studied at the level of scanning electron microscopy, since the morphological information about this organ are scarce in the literature available. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PARIZI, LUIS FERNANDO; RANGEL, CAROLINA KONRDORFER; SABADIN, GABRIELA ALVES; SAGGIN, BIANCA FAGUNDES; KIIO, IRENE; XAVIER, MARINA AMARAL; MATOS, RENATA DA SILVA; CAMARGO-MATHIAS, MARIA IZABEL; SEIXAS, ADRIANA; KONNAI, SATORU; et al. Rhipicephalus microplus cystatin as a potential cross-protective tick vaccine against Rhipicephalus appendiculatus. TICKS AND TICK-BORNE DISEASES, v. 11, n. 3, . (14/13143-7)
ARVEY RIVERA-PAEZ, FREDY; SAMPIERI, BRUNO RODRIGUES; LABRUNA, MARCELO BAHIA; MATOS, RENATA DA SILVA; MARTINS, THIAGO FERNANDES; CAMARGO-MATHIAS, MARIA IZABEL. Comparative analysis of germ cells and DNA of the genus Amblyomma: adding new data on Amblyomma maculatum and Amblyomma ovale species (Acari: Ixodidae). Parasitology Research, v. 116, n. 11, p. 2883-2892, . (14/13143-7, 12/02384-8)
MATOS, RENATA SILVA; DAEMON, ERIK; DE OLIVEIRA MONTEIRO, CAIO MARCIO; SAMPIERI, BRUNO RODRIGUES; MARCHESINI, PAULA BARROSO C.; DELMONTE, CAMILA; CAMARGO-MATHIAS, MARIA IZABEL. Thymol action on cells and tissues of the synganglia and salivary glands of Rhipicephalus sanguineus sensu lato females (Acari: Ixodidae). TICKS AND TICK-BORNE DISEASES, v. 10, n. 2, p. 314-320, . (14/13143-7)
MATOS, RENATA SILVA; DE OLIVEIRA, PATRICIA ROSA; COELHO, LETICIA; FERREIRA DE PAULA, LUIZA GABRIELLA; ZERINGOTA, VIVIANE; SILVA, BIANCA CARVALHO; MONTEIRO, CAIO; DAEMON, ERIK; CAMARGO-MATHIAS, MARIA IZABEL. Thymol: Effects on reproductive biology and Gene's organ morphology in Rhipicephalus sanguineus sensu lato engorged females (Acari: Ixodidae). TICKS AND TICK-BORNE DISEASES, v. 11, n. 1, . (14/13143-7)
RODRIGUES, LEANDRO; GIGLIOTI, RODRIGO; GOMES, ANA CAROLINA PERONI; KATIKI, LUCIANA MORITA; OTSUK, IVANI POZAR; DA SILVA MATOS, RENATA; NODARI, ELEN FERNANDA; VERISSIMO, CECILIA JOSE. In Vitro Effect of Volatile Substances from Eucalyptus Oils on Rhipicephalus microplus. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 30, n. 5, p. 737-742, . (14/02843-8, 14/13143-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)

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