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Molecular profile and phylogenetic analysis of Fusarium species recovered from air and isolated from blood cultures of immunocompromised patients

Grant number: 14/07403-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2014
Effective date (End): December 31, 2015
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Maria Luiza Moretti
Grantee:Pamella Stivanelli
Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


Fusarium species can cause invasive infections with high mortality rates in patients with severe and persistent neutropenia. From 2006 to 2013, 34 patients from Hospital de Clínicas - Unicamp were diagnosed with invasive fusariosis. The incidence density in our hospital was surprisingly higher than most hospitals worldwide. Considering that the quality of the environment can have a direct and significant influence on the occurrence of nosocomial infections, the aim of this study is to evaluate the hospital air as a potential environmental source of fusariosis in immunocompromised patients by molecular techniques. We will evaluate 32 isolates of Fusarium spp. recovered from hospital air (N=14) and patient's blood cultures (N=18) at Hospital de Clínicas, Unicamp, Campinas - SP. Fifteen clinical isolates and 13 environmental samples were identified as F. solani; three clinical samples and one environmental isolate were classified as F. napiforme. Fungi samples will be submitted to sequencing of ITS and D1/D2 regions of ribosomal DNA and haplotypes will be generated for each isolate by using MLST methodology. The evolutionary relatedness between clinical and air samples will be addressed with MEGA5 software ( By using this approach, we intend to establish a clonal relation for clinical and environmental isolates, as well as to confirm the hospital air as a source of Fusarium spp. infection in immunocompromised patients and stress the relevance of a controlled environment in health services. To the best of our knowledge, there are no studies describing a genetic relation between air and clinical samples of Fusarium spp.

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