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Emerging enteric viruses in wastewater from São Paulo city: Epidemiological surveillance

Grant number: 13/26586-1
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2014
Effective date (End): August 31, 2017
Field of knowledge:Health Sciences - Collective Health - Public Health
Principal researcher:Maria Ines Zanoli Sato
Grantee:Tatiana Prado
Home Institution: Companhia Ambiental do Estado de São Paulo (CETESB). Secretaria do Meio Ambiente (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Several infectious diseases can be transmitted through water contaminated with fecal material, including hepatitis and viral gastroenteritis. Among the enteric viruses, the most frequent target of research in the environment are norovirus (NoV), rotavirus species A (RV-A), human adenovirus (HAdV) and hepatitis A virus (HAV). Currently, the occurrence of other emerging viruses, such as polyomavirus (JCPyV) and hepatitis E virus (HEV) is also performed. The aim of this project is to establish a an environmental surveillance of these microorganisms in raw and treated sewage (including reclaimed water) from wastewater treatment plants (WWTPs) of the São Paulo city. Bacteriophage Bacteroides GB-124, potential indicator of the presence of these viruses in sewage, will also be analyzed by phage lyses technique. Secondary and tertiary treatment processes (filtration, ultra filtration and chlorination) will be evaluated for virus removal, allowing characterization of virological quality of effluents and the risks associated with reuse practices or disposal in surface water. Techniques of nucleic acid amplification and molecular characterization by nucleotide sequencing and phylogenetic analysis will be used for detection, quantification and viral genotyping. These results will provide relevant information about the epidemiology of enteric and emerging viruses (NoV GIV, and JCPyV HEV) in wastewater from São Paulo city Analytical protocols for viral detection will be tested and validated in order to provide useful diagnostic tools to assess emerging pathogens in the environment.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PRADO, TATIANA; BRUNI, ANTONIO DE CASTRO; FUNADA BARBOSA, MIKAELA RENATA; GARCIA, SUZI CRISTINA; MORENO, LUISA ZANOLLI; ZANOLI SATO, MARIA INES. Noroviruses in raw sewage, secondary effluents and reclaimed water produced by sand-anthracite filters and membrane bioreactor/reverse osmosis system. Science of The Total Environment, v. 646, p. 427-437, . (13/26586-1)
PRADO, TATIANA; BRUNI, ANTONIO DE CASTRO; FUNADA BARBOSA, MIKAELA RENATA; GARCIA, SUZI CRISTINA; DE JESUS MELO, ADALGISA MARIA; ZANOLI SATO, MARIA INES. Performance of wastewater reclamation systems in enteric virus removal. Science of The Total Environment, v. 678, p. 33-42, . (13/26586-1)
PRADO, TATIANA; BRUNI, ANTONIO DE CASTRO; FUNADA BARBOSA, MIKAELA RENATA; SANTOS BONANNO, VILMA MARQUES; GARCIA, SUZI CRISTINA; SATO, MARIA INES ZANOLI. Distribution of human fecal marker GB-124 bacteriophages in urban sewage and reclaimed water of Sao Paulo city, Brazil. JOURNAL OF WATER AND HEALTH, v. 16, n. 2, p. 289-299, . (13/26586-1)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.