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Histological analysis of osseointegration in autografts and allografts in rabbit tibiae

Grant number: 14/10981-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2014
Effective date (End): August 31, 2015
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Débora Pallos
Grantee:Keila Ilse Cangussu Brito
Host Institution: Universidade de Santo Amaro (UNISA). São Paulo , SP, Brazil


The aim of this study is to evaluate osseointegration in implants placed simultaneously with autogenous grafts and allogenic only type in the tibia of rabbits by histological analysis . Twelve New Zealand rabbits received implants installed with autogenous grafts (group1) and allograft (group 2) , the implants were installed in the iliac crest of rabbits that underwent two surgical procedures , with an interval of 18 days. The blocks are dehydrated in ethanol series (60 - 100%) and subsequently infiltrates and polymerized photopolymerizable resin. The blocks will be cut at a central point using a cutting and wear (Exakt Apparatebeau). The cut sections that make up the blades will be approximately 120 mm thick and stained with hematoxylin / eosin. The images will be captured and analyzed in a light microscope LEICA at 200x magnification. Histomorphometric evaluations will be performed by a trained and blinded examiner using ImageJ ® software for image analysis. The following parameters will be determine: bone-implant contact area (mm2) , bone area between the threads to each group . The data of bone-implant contact area (mm2) and bone area between the threads, will be tested by normality test - Kolmogorov - Smirnov. Test samples giving normality of t student test will be applied, and in cases of absence of normality, the Mann Whitney test will be used. The significance level is 5 %.

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