Histological analysis of osseointegration in autografts and allografts in rabbit tibiae

Abstract

The aim of this study is to evaluate osseointegration in implants placed simultaneously with autogenous grafts and allogenic only type in the tibia of rabbits by histological analysis . Twelve New Zealand rabbits received implants installed with autogenous grafts (group1) and allograft (group 2) , the implants were installed in the iliac crest of rabbits that underwent two surgical procedures , with an interval of 18 days. The blocks are dehydrated in ethanol series (60 - 100%) and subsequently infiltrates and polymerized photopolymerizable resin. The blocks will be cut at a central point using a cutting and wear (Exakt Apparatebeau). The cut sections that make up the blades will be approximately 120 mm thick and stained with hematoxylin / eosin. The images will be captured and analyzed in a light microscope LEICA at 200x magnification. Histomorphometric evaluations will be performed by a trained and blinded examiner using ImageJ ® software for image analysis. The following parameters will be determine: bone-implant contact area (mm2) , bone area between the threads to each group . The data of bone-implant contact area (mm2) and bone area between the threads, will be tested by normality test - Kolmogorov - Smirnov. Test samples giving normality of t student test will be applied, and in cases of absence of normality, the Mann Whitney test will be used. The significance level is 5 %.