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Effect of fractioned collection on sperm kinetics and viability of cooled and frozen stallion sêmen

Grant number: 14/00354-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Frederico Ozanam Papa
Grantee:Sidnei Nunes de Oliveira
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Several studies have been conducted and questioned about the real need of the seminal plasma on fertilization process. The existence of a significant variation in the refrigerated semen motility among stallions may in part be due to the different compositions of the seminal plasma. The seminal plasma is removed during the cryopreservation equine semen process due its negative effect on sperm viability. However most studies that relate to sperm survival during storage have been performed with whole ejaculate and there are few studies that assess the individual influences of the fluid produced by accessory sex glands on semen. The aim of this study is to evaluate and to compare the effects of fractionated semen collection on stallions classified as bad coolers and bad freezers and good coolers and good freezers on the survival, motility, plasma membrane integrity and mitochondrial activity of equine sperm before and after the cryopreservation process of cooling and freezing and to compare these data to conventional collection (whole ejaculate). Therefore is expected an improvement in the sperm quality on the sperm rich fraction when compared to the others, especially on the semen samples from stallions classified as bad coolers and bad freezers. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA, S. N.; DE ARAUJO, E. A. B.; SILVA, L. F. M. C.; HAYASHI, R. M.; MERENDA, V. R.; PAPA, F. O.. Fractionated semen collection as a diagnostic tool for reproductive pathologies in stallions. EQUINE VETERINARY EDUCATION, . (14/00354-0)
OLIVEIRA, SIDNEI N.; ANDRADE, JR., LUIZ R. P.; SILVA, LUIS F. M. C.; ARAUJO, ENDRIGO A. B.; RAYASHI, RAFAELLA M.; SEGABINAZZI, LORENZO G. T. M.; ALVARENGA, MARCO A.; DELL'AQUA, CAMILA P. F.; DELL'AQUA, JR., JOSE A.; PAPA, FREDERICO O.. Fractionated semen collection as a tool to rescue fertility in stallions with seminal vesiculitis. Theriogenology, v. 157, p. 110-120, . (14/00354-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
OLIVEIRA, Sidnei Nunes de. Effect the fractionated collection about kinetic and sperm viability the sperm cooled and frozen stallion. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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