This research project is aimed at fabricating layer-by-layer (LbL) films made with lactated-TMC [copolymer of poly(lactic acid) and N,N,N-trimethyl chitosan] and chondroitin sulfate (CS), for which different deposition methods will be used (dipping, spraying and spin-coating). Synergy will be sought on the properties of TMC, a cationic derivative obtained by quaternization of amino groups of chitosan (CHI), which is soluble in water and displays bactericidal effect, and of poly(lactic acid), a biodegradable polymer that is highly hydrolysable in the human body and used in the medical devices. The polyanion chondroitin sulfate (CS), a natural polysaccharide with excellent pharmaceutical properties, will be used for complexing to lactated-TMC. These (lactated-TMC)/(SC) LbL films may act as coatings for devices. The films will be tested for their antibacterial properties and cell proliferation using VERO and Caco-2 cells. The interactions between (lactated-TMC)/(CS) films with vitamin B12 will be studied in order to incorporate the latter vitamin in the films for controlled release. It will therefore be possible to assess the possible use of (lactated-TMC)/(CS) LbL films in coating biomedical devices.
News published in Agência FAPESP Newsletter about the scholarship: