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Hydrolysis of cane bagasse to hydrogen production in mesophilic and thermophilic conditions

Grant number: 13/22346-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2014
Effective date (End): July 31, 2017
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Maria Bernadete Amâncio Varesche
Grantee:Laís Américo Soares
Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:09/15984-0 - Bioenergy production from wastewaters and environmental fitting of liquid and solid wastes generated, AP.TEM


The lignocellulosic waste has been considered as an important substrate to biological hydrogen production, since it is widely produced, but subtilized. The sugarcane bagasse (BCA) could be used as carbon source in fermentative process. However, will be necessary the BCA pretreatment to available sugar to microorganism. In this work, the BCA will be used with and without steam explosion pretreatment. The mesophilic and thermophilic cellulolytic microbial community will be from soil samples and sludge from UASB reactor. The biomass will be enriched in an anaerobic cellulolytic medium. The assays will be performed in two steps: (I) BCA in nature will be pretreated by steam explosion to verify the effect of pretreatment on hydrogen production and (II) the better condition will be used in a new step where will be evaluated the effect of temperature and yeast extract on hydrogen production. Every assay will be realized in triplicates. As the hydrogen production as the other fermentation's products will be evaluated by gas and liquid chromatography, respectively. Microbial characterization from Bacteria Domain will be performed by microscopy and tools of molecular biology, like clone and 16S rRNA sequencing. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES, LAIS AMERICO; BORGES SILVA RABELO, CAMILA ABREU; SAKAMOTO, ISABEL KIMIKO; DELFORNO, TIAGO PALADINO; SILVA, EDSON LUIS; AMANCIO VARESCHE, MARIA BERNADETE. Metagenomic analysis and optimization of hydrogen production from sugarcane bagasse. BIOMASS & BIOENERGY, v. 117, p. 78-85, . (09/15984-0, 13/22346-6)
SOARES, LAIS AMERICO; SILVA RABELO, CAMILA ABREU B.; SAKAMOTO, ISABEL KIMIKO; SILVA, EDSON LUIS; VARESCHE, MARIA BERNADETE AMANCIO. Screening and Bioprospecting of Anaerobic Consortia for Biofuel Production Enhancement from Sugarcane Bagasse. Applied Biochemistry and Biotechnology, v. 190, n. 1, p. 232-251, . (15/06246-7, 09/15984-0, 13/22346-6)
SOARES, L. A.; RABELO, C. A. B. S.; DELFORNO, T. P.; SILVA, E. L.; VARESCHE, M. B. A.. Experimental design and syntrophic microbial pathways for biofuel production from sugarcane bagasse under thermophilic condition. RENEWABLE ENERGY, v. 140, p. 852-861, . (13/22346-6)
SOARES, LAIS AMERICO; BRAGA, JULIANA KAWANISHI; MOTTERAN, FABRICIO; SAKAMOTO, ISABEL KIMIKO; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Design and optimization of hydrogen production from hydrothermally pretreated sugarcane bagasse using response surface methodology. WATER SCIENCE AND TECHNOLOGY, v. 76, n. 1, p. 95-105, . (13/22346-6)
SOARES, LAIS AMERICO; BRAGA, JULIANA KAWANISH; MOTTERAN, FABRICIO; SAKAMOTO, ISABEL KIMIKO; SANTIAGO MONTEIRO, PATRICIA APARECIDA; SELEGHIM, JR., PAULO; AMANCIO VARESCHE, MARIA BERNADETE. Bioconversion of Sugarcane Bagasse into Value-Added Products by Bioaugmentation of Endogenous Cellulolytic and Fermentative Communities. WASTE AND BIOMASS VALORIZATION, v. 10, n. 7, p. 1899-1912, . (09/15984-0, 13/22346-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOARES, Laís Américo. Anaerobic bioconversion of sugarcane bagasse in biotechnological products in thermophilic and mesophilic condition. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) São Carlos.

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