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Development and application of method for silver determination in the hemolymph of amphipods exposed to nanomaterials

Grant number: 13/26301-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2014
Effective date (End): June 30, 2018
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Gisela de Aragão Umbuzeiro
Grantee:Monizze Vannuci e Silva
Home Institution: Faculdade de Tecnologia (FT). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Associated scholarship(s):16/19635-4 - Assessment of behavioural and reproductive effects in marine amphipods exposed to silver, BE.EP.DR


Silver has been largely incorporated in the nanomaterials due to its broad-spectrum antimicrobial action. Currently, the silver nanoparticles (AgNP) are being released into the environment in amounts that are becoming an environmental concern. Studies about AgNP discuss whether the toxicity of this nanomaterial is due the ionic silver dissolution or if the nanoparticles themselves are the responsible by the high toxicity. The methodological difficulty in the Ag determination in biological materials and in low concentrations, mainly in saline samples (marine environment), is a limiting factor for toxicological studies with this metal. In this work, will be developed and evaluated a method for the determination of silver in the Brazilian marine amphipod, Parhyale hawaiensis, exposed to nanoparticles of this metal through feeding. Besides that, copper concentration, which is naturally present in the hemolymph of these animals, will be used as an intern standard. Parhyale hawaiensis individuals will be exposed to AgNP incorporated in the food and, after that, dissection and separation of hemolymph and organs/tissues of the exposed individuals will be done. The procedures of biological material digestion and interferences elimination will be evaluated, aiming the determination by atomic spectrometry (absorption and/or emission). Concentrations of Ag and Cu and the Ag distribution in the different fractions of the biological material will be determined. Also, the seawater will be analyzed. From the results, it is expected to obtain information about the absorption, destination and storage of silver in the amphipod, providing data for the development of biomarkers of exposure. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VANNUCI-SILVA, MONIZZE; KOHLER, SHANELLE; UMBUZEIRO, GISELA DE A.; FORD, ALEX T. Behavioural effects on marine amphipods exposed to silver ions and silver nanoparticles. Environmental Pollution, v. 252, n. B, p. 1051-1058, SEP 2019. Web of Science Citations: 0.
VANNUCI-SILVA, MONIZZE; CADORE, SOLANGE; HENRY, THEODORE B.; UMBUZEIRO, GISELA. Higher silver bioavailability after nanoparticle dietary exposure in marine amphipods. Environmental Toxicology and Chemistry, v. 38, n. 4, p. 806-810, APR 2019. Web of Science Citations: 1.
VANNUCI-SILVA, MONIZZE; DA SILVA, EMANUELI DO NASCIMENTO; ARTAL, MARIANA COLETTY; DOS SANTOS, AMANDA; SILVA, FABIO; UMBUZEIRO, GISELA DE A.; CADORE, SOLANGE. GFAAS and ICP-MS Determination of Ag and Cu in the Haemolymph of a Millimetric Marine Crustacean (Parhyale hawaiensis) as a Tool in Ecotoxicology. ATOMIC SPECTROSCOPY, v. 39, n. 2, p. 67-74, MAR-APR 2018. Web of Science Citations: 4.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Monizze Vannuci e. Silver nanoparticles and silver salts : internal concentration and behavioural effects on marine amphipods. 2018. Doctoral Thesis - Universidade Estadual de Campinas, Instituto de Biologia.

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