Role of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic exercise in an experimental model of asthma

Abstract

Asthma is a chronic airway inflammatory disease that results in impairment of quality of life. In the last years, several studies have demonstrated that Aerobic Training (AT) performed properly results in anti-inflammatory effects to the airways, either in experimental models of asthma and to asthmatic individuals. However, although few cellular effects have been investigated (still only in animals' models), the possible extra and intracellular mediators underlying the effects of AT on asthma never were investigated, except to NF-kB. Thus, the present project aims to evaluate the effects of AT on SOCS, JAK and STAT proteins signaling on the lungs as possible modulators of the anti-inflammatory effects of aerobic training in an experimental model of asthma (by using House Dust Mite - HDM). For this purpose, the animals will receive intra-tracheal administration of HDM (100ug) on days 0, 7, 14, 21, 28, 35 e 42 and will be submitted to AT (for evaluation of therapeutic effect) begining on day 17 during 5 weeks. Twenty-four hours after the last session of training and allergen challenge, the animals will be assessed to the inflammatory profile in the bronchoalveolar lavage and in the lung tissue, to the levels of pro-inflammatory cytokines (IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-12, IL-13, IL-17, IL-23) and to the levels of anti-inflammatory cytokines (IL-10, IL-1ra) and also for the expression of SOCS1, SOCS2, SOCS3, JAK2, STAT3, STAT5 e STAT6 in the lungs, as well as for the airway remodeling, and bronchial hyperresponsiveness. (AU)