Candida albicans biofilm on antimicrobial resin for dental prosthesis using dual-flow cell system

Abstract

The continuous use of removable dentures can foster oral infectious diseases such as denture stomatitis, which is associated to precarious oral hygiene habits and denture biofilm. The incorporation of an antiseptic agent in denture bases has great preventive and therapeutic potentials against such pathologic processes, although it needs further investigation regarding its feasibility. The aim of this study is to evaluate the effect of incorporating cetylpyridinium chloride (CPC) on a base acrylic resin on the biofilm formation of Candida albicans, using dual-flow cell system method. For this purpose, disc-shaped specimens (n=10 for each concentration) will be made of acrylic resin for the colorless denture base, containing 0 (control), 0.1 or 1 g of CPC and another with 50% of methacrylic acid (MA). The specimens will present 50% of MA to replace the monomer. Before biofilm formation, sterile resin discs will be immersed in sterile human saliva for 1 hour and suspended C. albicans (1.0 x 107 cells/mL) will be injected in the flow chamber for initial adherence. After 2 h, a 0.2 mL/min flow rate of sterile broth will start, leading to a shear rate of (9.65 s-1), which is compatible with fluid flow in the oral cavity. Comparison of biofilm formation considering the different concentrations of CPC will be performed in real time by a dark field microscope and confocal microscope. (AU)