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Biotechnological production and evaluation of biosurfactants properties by Lactococcus lactis CECT-4434 from agro-industrial wastes

Grant number: 13/15065-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2014
Effective date (End): June 30, 2017
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Technology
Principal Investigator:Ricardo Pinheiro de Souza Oliveira
Grantee:Ellen Cristina Souza Vera
Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Biosurfactants are surfactants that have a biological origin. It is a group of structurally diverse molecules tensoactive, who usually have lower toxicity and greater biodegradability than the synthetic surfactants. The most extensively studied applications of biosurfactants are those related to the petroleum industry and the biorremediacao of locations or waste contaminated with hydrocarbons, although in recent years, has increased the interest in evaluating the potential usefulness of biosurfactants as additives in the areas of food, pharmaceutical, medical and veterinary (given that there are studies that demonstrate properties antibacterial, antifungal, antiviral, antitumor and even to combat the accession and formation of biofilms of pathogenic micro-organisms). Thus, the present project has as main objective the use of agro-industrial residues (whey and vinasse from sugar cane) as renewable sources for the biotechnological production of biosurfactants, employing the strain Lactococcus lactis CECT-4434 and subsequent evaluation of their properties as additives. The project will be developed in 2 stages, being the 1st step will be developed in Brazil (University of São Paulo) and the 2nd step in Spain (University de Vigo). Brief Overview of the activities that will be developed are: Brazil - Evaluate the influence of the whey and stillage to formulate culturemedia alternative and natural; studying the influence of alternative sources of carbon and nitrogen; assess, with the aid of a fractionated factorial planning the influence of relevant variables in the cultivations conducted in metabolic shaker; assess the kinetics of the process in metabolic shaker; evaluate, through an experimental planning DCCR (Central Composite Design Rotational), in bioreactor, the influence of the factors (agitation and aeration), expressed by volumetric coefficient of oxygen transfer (kLa); evaluate the kinetics of the process in bioreactor 3 liters; and recover, isolate and analyze the biosurfactants during the cultivations in metabolic shaker and in bioreactor of bench. Spain - Studies of physico-chemical characterisation of biosurfactants; stability of biosurfactants in extreme conditions of pH and temperature; assess the properties of biosurfactants as additives. For this reason, will be studied the antimicrobial capacity of biosurfactants face many pathogenic micro-organisms, the ability of biosurfactants to form emulsions and the inhibition of adhesion of pathogenic micro-organisms to solid surfaces when using biosurfactants.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUZA, ELLEN CRISTINA; DE SOUZA DE AZEVEDO, PAMELA OLIVEIRA; DOMINGUEZ, JOSE MANUEL; CONVERTI, ATTILIO; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Influence of temperature and pH on the production of biosurfactant, bacteriocin and lactic acid by Lactococcus lactis CECT-4434. CYTA-JOURNAL OF FOOD, v. 15, n. 4, p. 525-530, . (13/15065-0)
SOUZA VERA, ELLEN CRISTINA; DE SOUZA DE AZEVEDO, PAMELA OLIVEIRA; DOMINGUEZ, JOSE MANUEL; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Optimization of biosurfactant and bacteriocin-like inhibitory substance (BLIS) production by Lactococcus lactis CECT-4434 from agroindustrial waste. Biochemical Engineering Journal, v. 133, p. 168-178, . (13/15065-0)
SOUZA, E. C.; VESSONI-PENNA, T. C.; AL ARNI, S.; DOMINGUEZ, J. M.; CONVERTI, A.; OLIVEIRA, R. P. DE S.. Influence of toluene and salinity on biosurfactant production by Bacillus sp.: scale up from flasks to a bench-scale bioreactor. Brazilian Journal of Chemical Engineering, v. 34, n. 2, p. 395-405, . (13/15065-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VERA, Ellen Cristina Souza. Biotechnological production of biosurfactant by Lactococcus lactis CECT- 4434 from agroindustrial residues and evaluation of its properties. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

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