Toxoplasmosis is a disease resulting from infection by the parasite Toxoplasma gondii that affects much of the world's population. Clinical manifestations in brain, congenital and ocular forms. The prevalence of this disease varies in different areas around the world and depends on climatic factors, eating habits, hygiene and age of affected individuals. In recent years our group has shown that the incidence of infection by T. gondii in pregnant women from the northwest region of the state of São Paulo are approximately 65.0 % , the rate of congenital infection in São José do Rio Preto, SP is 2.3 % . The genetic characterization of T. gondii performed on human samples revealed a great diversity and so we try to clarify the relationship between different strains and human infection, including our region. The aim of this study is to characterize the infection by T. gondii from human biological samples from pregnant women and newborns. Specific objectives include genotypically characterized strains of T. gondii in the region using the method of PCR - RFLP and establish relationship with the clinical picture of affected patients. Serum and genomic DNA of patients with suspected or clinical diagnosis of gestational and congenital toxoplasmosis will be selected, stored at the Laboratory of Immunogenetics , Department of Molecular Biology FAMERP , whose epidemiological data of the same patients were collected . Commercial ELISA methods (DiaSorin) and ELFA (Biomerieux) will be used for the identification of IgA, IgM and IgG anti -T. gondii. The identification of the genomic DNA of T. gondii is performed with the use of conventional PCR methods, nested PCR, Real Time PCR and PCR- RFLP. The results of serological and molecular analyzes will be compared. Statistical tests Ç2 (with Yates correction), Fisher 's exact test and t test ( if applicable ) will be used in comparisons. You will also calculated the odds ratio and confidence interval of 95 %, when necessary. The results of this project will contribute to the knowledge of the molecular epidemiology of such important forms of toxoplasmosis, which is the gestational and congenital, associating them with disease severity. Furthermore, they may contribute to the understanding of congenital infection and outbreak of toxoplasmosis in fetuses and newborns.
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