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Maternal high fat diet effects on neuronal fate of arcuate nucleus during development

Grant number: 14/06980-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): August 01, 2014
Effective date (End): July 31, 2015
Field of knowledge:Biological Sciences - Pharmacology
Principal researcher:Luciano Freitas Felicio
Grantee:Marianne Orlandini Klein
Supervisor abroad: Alfonso Abizaid
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Carleton University, Canada  
Associated to the scholarship:12/07378-6 - Maternal high fat diet: influences on maternal behavior and offspring development, BP.DR


Developmental programming is influenced by perinatal nutrition and it has a long-lasting impact on adult metabolism in the offspring. In particular, maternal high fat diet has been associated with increased risk of obesity and metabolic disorders in the offspring. These effects may be due to the effects of the high fat diet on the development of the systems that regulate food intake and energy balance in the hypothalamus. The arcuate nucleus (ARC) of hypothalamus may be a particularly sensitive region as this nucleus contains cells that are sensitive to nutrients like glucose and fatty acids, and contains the POMC and AgRP/NPY neurons that play an important role on the control of energy balance and food intake. Thus, the aim of this study is to investigate the effects of maternal high fat diet during pregnancy on the development of the offspring ARC as a potential mechanism that results in altered neuronal fate of POMC and NPY neurons. To this end, we will investigate the effects of a high fat diet fed to mothers throughout pregnancy on the expression of neurogenin 3 (Ngn 3), a progenitor transcription factor important for the fate of POMC and NPY neurons within the ARC during development. Pregnant females will be assigned either to: high fat group (HF, 45% fat diet since day 0 of pregnancy) or control group (CON, standard diet). Pregnant females will be sacrificed on days 10, 13, 15 and 17 of pregnancy to obtain the brains from developing fetuses. It will be determined by immunofluorescence and in situ hybridization the differences in the expression of Ngn 3, POMC and NPY at all time points. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
KLEIN, MARIANNE ORLANDINI; MACKAY, HARRY; EDWARDS, ALEXANDER; PARK, SU-BIN; INHASZ KISS, ANA CAROLINA; FELICIO, LUCIANO FREITAS; ABIZAID, ALFONSO. POMC and NPY mRNA expression during development is increased in rat offspring brain from mothers fed with a high fat diet. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, v. 64, n. SI, p. 14-20, . (14/06980-0, 12/07378-6)

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