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Development of a 3D oral mucosa model for in vitro studies

Grant number: 14/06057-7
Support Opportunities:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): August 01, 2014
Effective date (End): October 31, 2014
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Josimeri Hebling Costa
Grantee:Fernanda Gonçalves Basso
Supervisor: Stephen E. Feinberg
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Research place: University of Michigan, United States  


Several studies have been developed to evaluate the oral mucosa repair process. So far, most of the in vitro studies were performed using two dimensional cell culture methods, where cells are seeded in a monolayer model. These studies have provided great contributions about cell behavior at normal conditions and in response to different stimuli. However, the two dimensional cell culture models show some limitations, such as poor cell interactions to extracellular matrix and the interactions of different cell lines. To overcome these limitations, the three dimensional (3D) cell culture models were developed, to better simulate the in vivo conditions of cells interactions and phenotypes expression. To date, the development and standardization of a 3D oral mucosa model containing stratified epithelium and cellularized connective tissue would not only improve the comprehension of cells interactions, but also the elucidation of the etiopathogenesis of several oral conditions, such as oral mucositis, improving also the treatment strategies and protocols. The aim of this study will be to develop a 3D oral mucosa equivalent model (EVPOME), using an AlloDerm acellular matrix, for in vitro studies. The development of this model, that shows similar morphology and phenotype as oral mucosa will enlarge the use of 3D oral mucosa model for elucidation of several oral conditions and treatment protocols. Oral keratinocytes and fibroblasts will be obtained from healthy young individuals and 3D cell culture model will be obtained using the AlloDerm scaffold for fibroblasts cell culture, followed by the keratinocyte cell culture. Cell viability, cell culture morphology and cell proliferation will be evaluated by MTT Assay, H&E staining and PCNA immunohistochemistry, respectively. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASSO, FERNANDA G.; PANSANI, TAISA N.; MARCELO, CYNTIA L.; DE SOUZA COST, CARLOS ALBERTO; HEBLING, JOSIMERI; FEINBERG, STEPHEN E.. Phenotypic markers of oral keratinocytes seeded on two distinct 3D oral mucosa models. TOXICOLOGY IN VITRO, v. 51, p. 34-39, . (13/05879-0, 12/17947-8, 14/06057-7)
BASSO, F. G.; HEBLING, J.; MARCELO, C. L.; COSTA, C. A. DE SOUZA; FEINBERG, S. E.. Development of an oral mucosa equivalent using a porcine dermal matrix. BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY, v. 55, n. 3, p. 308-311, . (12/17947-8, 14/06057-7, 13/05879-0)
BASSO, FERNANDA G.; PANSANI, TAISA N.; SOARES, DIANA G.; SCHEFFEL, DEBORA L.; BAGNATO, VANDERLEI S.; DE SOUZA COSTA, CARLOS ALBERTO; HEBLING, JOSIMERI. Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy. Photochemistry and Photobiology, v. 91, n. 4, p. 952-956, . (13/05879-0, 12/17947-8, 14/06057-7)
BASSO, FERNANDA G.; PANSANI, TAISA N.; SOARES, DIANA G.; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. LLLT Effects on Oral Keratinocytes in an Organotypic 3D Model. Photochemistry and Photobiology, v. 94, n. 1, p. 190-194, . (14/06057-7, 13/05879-0, 12/17947-8)

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