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Hydrolysis of sugar cane bagasse for the hydrogen and biogas production with suspended and immobilized in nanocoatings biomass

Grant number: 13/20196-7
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): May 01, 2014
Effective date (End): February 28, 2018
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Maria Bernadete Amâncio Varesche
Grantee:Juliana Kawanishi Braga
Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:09/15984-0 - Bioenergy production from wastewaters and environmental fitting of liquid and solid wastes generated, AP.TEM
Associated scholarship(s):14/11851-4 - Hydrolysis of sugar cane bagasse for the hydrogen production in mesophilic condition, BE.EP.PD


Lignocellulosic residues such as sugar cane bagasse are abundant sources of carbohydrates and their bioconversion has received great attention. It is known that the biological production of hydrogen and biogas makes sugar cane economically competitive since is low cost source of carbohydrates. Whereas the São Paulo state is the largest processor of sugarcane in Brazil and that this activity generates large amounts of waste rich in carbohydrates, especially sugar cane bagasse, this study will be evaluated its use as a substrate for the hydrogen and biogas (CH4 and CO2) production. For this project implementation we intend to conduct the bagasse hydrolysis using the fungus Aspergillus niger (stage 1), as well as the steam explosion. Tests in fermentative condition for H2 production (stage 2), and methanogenic-fermentative condition for biogas production (stage 3) will be performed with the hydrolyzed sugar cane bagasse and the residue from the end of the stage 2 respectively. The inocula for methanogenic-fermentative and fermentative tests will be from different origins, that is, in the first case fermentative bacteria enriched biomass will be used. In the second case inoculum from a mesophilic UASB reactor treating poultry DAKAR (Tiête - SP) will be applied. Synthetic substrate assay similar to stage 2 will be used in H2 production assay with immobilized bacteria in nanocoatings in a continuous flow reactor (stage 4). Finally microbial characterization will be performed by microbiological microscopy and molecular biology (PCR / DGGE of 16S rRNA sequencing and pyrosequencing). (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRAGA, JULIANA KAWANISHI; STANCARI, RAFAELA ARANTES; MOTTERAN, FABRICIO; MALAVAZI, IRAN; VARESCHE, MARIA BERNADETE AMANCIO. etals addition for enhanced hydrogen, acetic and butyric acids production from cellulosic substrates by Clostridium butyricu. BIOMASS & BIOENERGY, v. 150, . (09/15984-0, 13/20196-7)
BRAGA, JULIANA K.; ABREU, ANGELA A.; MOTTERAN, FABRICIO; PEREIRA, MARIA ALCINA; ALVES, MARIA MADALENA; VARESCHE, MARIA BERNADETE A.. Hydrogen Production by Clostridium cellulolyticum a Cellulolytic and Hydrogen-Producing Bacteria Using Sugarcane Bagasse. WASTE AND BIOMASS VALORIZATION, v. 10, n. 4, p. 827-837, . (13/20196-7)
BRAGA, JULIANA K.; MOTTERAN, FABRICIO; SAKAMOTO, ISABEL K.; VARESCHE, MARIA BERNADETE A.. Bacterial and archaeal community structure involved in biofuels production using hydrothermal- and enzymatic-pretreated sugarcane bagasse for an improvement in hydrogen and methane production. SUSTAINABLE ENERGY & FUELS, v. 2, n. 12, p. 2644-2660, . (09/15984-0, 13/20196-7)
BRAGA, JULIANA K.; SOARES, LAIS A.; MOTTERAN, FABRICIO; SAKAMOTO, ISABEL KIMIKO; VARESCHE, MARIA BERNADETE A.. Effect of 2-bromoethanesulfonate on anaerobic consortium to enhance hydrogen production utilizing sugarcane bagasse. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 41, n. 48, p. 22812-22823, . (13/20196-7)

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