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Sequential extraction of bioactive compounds from passion fruit (Passiflora edulis f. flavicarpa) industrial byproducts using pressurized fluids

Grant number: 14/00372-8
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2014
Effective date (End): August 31, 2016
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Julian Martínez
Grantee:Juliane Viganó
Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


This work aims combining sequential procedures to maximize the extraction of bioactive compounds from passion fruit (Passiflora edulis f. flavicarpa) industrial byproducts. The relevance of this research is based on two common issues on the pulp/juice industry: waste reuse for adding value to it and development of clean techniques for the extraction of bioactive compounds from vegetal matter. The waste, namely, shell and pulp fiber will be acquired from the industry. Then, in the Laboratory of High Pressure in Food Engineering, the shell and pulp fiber will be dried at 50 ºC, milled and analyzed in terms of proximate composition. The particles and extraction bed characteristics will be measured, such as: particle size, real density, apparent density and porosity. The dehydrated raw material will be subjected to three extraction methods: low pressure extraction (Soxhlet), and the processes based on extraction with pressurized fluids, namely, supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE). The high pressure extractions will be performed in two steps: (a) SFE and PLE isolated, (b) SFE and PLE sequentially. As solvents, carbon dioxide (CO2) will be used in SFE and different compositions of ethanol and water in PLE. Both techniques will be studied based on a central composite design for two factors with four replications for the central point. The factors are temperature (35-65 °C) and pressure (15-35 MPa) in the SFE and temperature (30-100 °C) and ethanol percentage in water (0-100 %, v/v) in the PLE. The extracts will be analyzed in terms of global yield, total phenolics, antioxidant activity and characterization of phenolics, carotenoids and fatty acids. Based on the retrieved results from the single extractions, a sequence of procedures will be performed using the same extraction bed, yielding different extracts from the raw material. The extracts of this step will be analyzed in the same way of the previous extractions. Finally, the sequential extraction kinetics will be studied by fitting descriptive models to the experimental data. In order to assess the extraction effects on the material, scanning electronic microscopy can be performed on the particles before and after the extractions. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VIGANO, JULIANE; COUTINHO, JANCLEI P.; SOUZA, DANILO S.; BARONI, NAIARA A. F.; GODOY, HELENA T.; MACEDO, JULIANA A.; MARTINEZ, JULIAN. Exploring the selectivity of supercritical CO2 to obtain nonpolar fractions of passion fruit bagasse extracts. JOURNAL OF SUPERCRITICAL FLUIDS, v. 110, p. 1-10, . (14/00372-8, 13/02203-6)
VIGANO, JULIANE; BRUMER, ISABELA ZABOTI; DE CAMPOS BRAGA, PATRICIA APARECIDA; DA SILVA, JULIANA KELLY; MAROSTICA JUNIOR, MARIO ROBERTO; REYES REYES, FELIX GUILLERMO; MARTINEZ, JULIAN. Pressurized liquids extraction as an alternative process to readily obtain bioactive compounds from passion fruit rinds. FOOD AND BIOPRODUCTS PROCESSING, v. 100, n. A, p. 382-390, . (14/00372-8, 15/11932-7, 13/02203-6)
VIGANO, JULIANE; DA FONSECA MACHADO, ANA PAULA; MARTINEZ, JULIAN. Sub- and supercritical fluid technology applied to food waste processing. JOURNAL OF SUPERCRITICAL FLUIDS, v. 96, n. SI, p. 272-286, . (14/00372-8, 13/02203-6)
VIGANO, JULIANE; AGUIAR, ANA C.; MORAES, DAMILA R.; JARA, JOSE L. P.; EBERLIN, MARCOS N.; CAZARIN, CINTHIA B. B.; MAROSTICA JUNIOR, MARIO R.; MARTINEZ, JULIAN. Sequential high pressure extractions applied to recover piceatannol and scirpusin B from passion fruit bagasse. Food Research International, v. 85, p. 51-58, . (14/00372-8, 13/02203-6)
DOS SANTOS, PHILIPE; DE AGUIAR, ANA C.; VIGANO, JULIANE; BOEING, JOANA SCHUELTER; VISENTAINER, JESUI V.; MARTINEZ, JULIAN. Supercritical CO2 extraction of cumbaru oil (Dipteryx alata Vogel) assisted by ultrasound: Global yield, kinetics and fatty acid composition. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 75-83, . (14/00372-8, 13/02203-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VIGANÓ, Juliane. Extração sequencial de compostos bioativos de subprodutos industriais de maracujá amarelo (Passiflora edulis sp.) utilizando fluidos pressurizados. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.

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