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Bioactivity of experimental scaffolds of chitosan and collagen on cultured human dental pulp cells

Grant number: 13/23520-0
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): April 01, 2014
Effective date (End): March 31, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Carlos Alberto de Souza Costa
Grantee:Diana Gabriela Soares dos Passos
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):14/13034-3 - Odontoblastic differentiation and mineralized matrix deposition by dental pulp stem cells seeded on nanofibrous scaffolds associated to bioactive substances, BE.EP.PD


Direct pulp capping (DPC) remains as a limited clinical therapy. This is because the current capping agents cause superficial coagulation necrosis after being applied on the pulpal wound. However, pulp regeneration mediated by pulp cells takes place with time. In this study, a new biomaterial (scaffold of chitosan/collagen) for DPC will be tested. It will be assessed the cytotoxicity of this specific scaffold (spongy and injectable characteristics) as well as its ability of inducing human dental pulp cells (HDPCs) to synthesize and deposit mineralized matrix. Both scaffolds will be associated to different bioactive substances (simvastatin, vitamin D3, and TGF-ß), and the following parameters will be evaluated: active cell migration (trans-wells), cell viability (Alamar Blue/Live Dead), cell migration into scaffold (light microscopic/SEM), cell differentiation (ALP activity and Real Time PCR) and mineralized matrix deposition (Alizarin Red). First of all, the cytotoxicity of the scaffolds will be evaluated on HDPCs seeded on them, and the cell viability and migration scaffolds will be analyzed. Then, the bioactive concentration of each substance capable of increasing all the cell parameters tested, will be determinate. Finally, the scaffolds plus substances will be evaluated, and all the cell parameters will be tested again. The quality of mineralized matrix will be assessed by micro-CT using a tooth slice model, on which the scaffolds will be adapted and the HDPCs seeded. Numerical data obtained will be submitted to specific statistical analysis.

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Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROBERTI GARCIA, LUCAS DA FONSECA; VOLTOLINI PONTES, ELAINE CRISTINA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; SOARES, DIANA GABRIELA. Transdentinal cytotoxicity of resin-based luting cements to pulp cells. CLINICAL ORAL INVESTIGATIONS, v. 20, n. 7, p. 1559-1566, . (13/23520-0, 13/05879-0, 12/14912-9)
SOARES, D. G.; BRITO, C. A.; TAVARES DA SILVA, R. H. B.; RIBEIRO, A. P. D.; HEBLING, J.; DE SOUZA COSTA, C. A.. Cytocompatibility of HEMA-free resin-based luting cements according to application protocols on dentine surfaces. International Endodontic Journal, v. 49, n. 6, p. 551-560, . (13/23520-0, 13/05879-0)
SOARES, DIANA GABRIELA; ROSSETO, HEBERT LUIS; BASSO, FERNANDA GONCALVES; SCHEFFEL, DEBORA SALLES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells. Brazilian Oral Research, v. 30, n. 1, . (14/13034-3, 13/23520-0)
SOARES, DIANA GABRIELA; SACONO, NANCY TOMOKO; RIBEIRO, ANA PAULA DIAS; BASSO, FERNANDA GONCALVES; SCHEFFEL, DEBORA SALES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Responses of Dental Pulp Cells to a Less Invasive Bleaching Technique Applied to Adhesively Restored Teeth. Journal of Adhesive Dentistry, v. 17, n. 2, p. 155-161, . (13/23520-0)
FERNANDA DA SILVEIRA VARGAS; DIANA GABRIELA SOARES; FERNANDA GONÇALVES BASSO; JOSIMERI HEBLING; CARLOS ALBERTO DE SOUZA COSTA. Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide. Brazilian Dental Journal, v. 25, n. 5, p. 367-371, . (13/23520-0)
SOARES, DIANA G.; BASSO, FERNANDA G.; SCHEFFEL, DEBORA SALLES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS A.. Responses of human dental pulp cells after application of a low-concentration bleaching gel to enamel. ARCHIVES OF ORAL BIOLOGY, v. 60, n. 9, p. 1428-1436, . (13/23520-0, 13/05879-0)
SOARES, DIANA G.; ANOVAZZI, GIOVANNA; BORDINI, ESTER ALVES F.; ZUTA, UXUA O.; SILVA LEITE, MARIA LUISA A.; BASSO, FERNANDA G.; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS A.. Biological Analysis of Simvastatin-releasing Chitosan Scaffold as a Cell-free System for Pulp-dentin Regeneration. JOURNAL OF ENDODONTICS, v. 44, n. 6, p. 971-976, . (13/23520-0, 16/15674-5)
SOARES, DIANA GABRIELA; ROSSETO, HEBERT LUIS; SCHEFFEL, DEBORA SALLES; BASSO, FERNANDA GONCALVES; HUCK, CLAUDIA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold. CLINICAL ORAL INVESTIGATIONS, v. 21, n. 9, p. 2827-2839, . (14/13034-3, 13/23520-0)
SOARES, DIANA GABRIELA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Effect of hydrogen-peroxide-mediated oxidative stress on human dental pulp cells. Journal of Dentistry, v. 43, n. 6, p. 750-756, . (13/23520-0)
SOARES, D. G.; MARCOMINI, N.; BASSO, F. G.; PANSANI, T. N.; HEBLING, J.; DE SOUZA COSTA, C. A.. Influence of Restoration Type on the Cytotoxicity of a 35% Hydrogen Peroxide Bleaching Gel. Operative Dentistry, v. 41, n. 3, p. 293-304, . (13/23520-0)

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