This study will be addressed anaerobic degradation of anionic and nonionic surfactants in commercial laundry wastewater, respectively, linear alkylbenzene sulfonate (LAS) and linear alcohol ethoxylate (LAE), both widely used in the manufacture of detergents and present in sewage and water industrial wastewater. In a certain way they may negatively affect the process of wastewater treatment, either with foam formation or with inhibition of organisms responsible for biological treatment through by-products generated from the surfactant`s degradation. An anaerobic fluidized bed reactor in pilot scale will be used filled with sand as support material. Previously the reactor operation, test of methanogenic potential, toxicicity and sludge choose with better removal efficiency of surfactants, will be performed in batch. The anaerobic fluidized bed reactor in pilot scale will be inoculated with sludge from UASB reactors containing high diversity of microorganisms. The reactor will be operated in 5 stages being (inoculation, adaptation, and stages with commercial laundry wastewater). Physicochemical analyzes of pH, alkalinity, COD, determination of LAS and LAE (through mass spectrometry LC/MS) and volatile organic acids (HPLC) for monitoring the influent and effluent of the reactor will be performed. For the phylogenetic characterization, pyrosequencing analysis of V3 region of 16S RNAr in each reactor operation stage will be carried out. In biological treatment processes the reactor's scale has fundamental importance. The difficulty in scaling up biotechnological processes is the maintenance of stability operating conditions, especially in anaerobic fluidized bed reactor, keep the effective mass transfer, fluidize the bed and hold the biomass attached to the support material. Currently, studies with removal and degradation of surfactants in anaerobic fluidized bed reactor on a bench scale are well consolidated. Thus, this study can fill gaps with regard to anaerobic degradation of anionic and nonionic surfactants present in real wastewater in a fluidized bed reactor in pilot scale as well as evaluating both, by-products generated through the degradation of these compounds and the microbial diversity present in this reactor model.
News published in Agência FAPESP Newsletter about the scholarship: