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Effects of different irrigation regimes in physico-chemical properties of dentin and consequences of changesin the adhesion of microorganisms and AH Plus sealer

Grant number: 13/19789-3
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2014
Effective date (End): October 31, 2017
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Clovis Monteiro Bramante
Grantee:Talita Tartari
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):15/09958-8 - Effect of different irrigation protocols on the attachment and differentiation of stem cells from the apical papilla, BE.EP.DR


The chemical solutions used to assist the endodontic instruments in the cleanse of the root canal system cause changes in the physicochemical properties of dentin, which may affect the adhesion of sealers and microorganisms. The objective of this study is to evaluate the effects of different irrigation regimes in physico-chemical properties of dentin and the consequences of these changes in adherence of microorganisms and AH Plus sealer. Fragments of root dentine will be subjected to photoacoustic Fourier-transform infrared spectroscopy (PA-FTIRS) for determining the changes in the components of the dentin and the time required for sodium hypochlorite (NaOCl) to deproteinate the collagen matrix exposed by chelating agents. The same specimens will be submitted to the scanning electron microscopy (SEM) for surface characterization and to the energy-dispersive X-ray spectroscopy (EDS) for determination of dentin components. New specimens will be prepared to assess changes in roughness by profilometer. Following, the dentin wettability will be determined by sessile drop technique and finally, these specimens will be subjected to the adhesion test of Enterococcus faecalis and Candida albicans in confocal laser scanning microscopy (CLSM). Lastly, transverse disks of roots will be obtained to evaluate the adhesion of AH Plus sealer by a push-out test and the sealer penetration in dentinal tubules by SEM and CLSM. After the adhesion test, the discs will be sectioned to determine the type of sealer fracture in a stereomicroscope. After each analysis, statistical tests will be conducted and then multiple linear regression analysis will be applied to correlate changes and dentin surface characteristics with sealer and microorganisms adhesion.

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TARTARI, T.; BACHMANN, L.; ZANCAN, R. F.; VIVAN, R. R.; DUARTE, M. A. H.; BRAMANTE, C. M.. Analysis of the effects of several decalcifying agents alone and in combination with sodium hypochlorite on the chemical composition of dentine. International Endodontic Journal, v. 51, n. 1, SI, p. E42-E54, . (13/19789-3, 12/02460-6)
TARTARI, T.; GUIMARAES, B. M.; AMORAS, L. S.; DUARTE, M. A. H.; SILVA E SOUZA, P. A. R.; BRAMANTE, C. M.. Etidronate causes minimal changes in the ability of sodium hypochlorite to dissolve organic matter. International Endodontic Journal, v. 48, n. 4, p. 399-404, . (13/19789-3, 12/06738-9)
TARTARI, T.; ODA, D. F.; ZANCAN, R. F.; DA SILVA, T. L.; DE MORAES, I. G.; DUARTE, M. A. H.; BRAMANTE, C. M.. Mixture of alkaline tetrasodium EDTA with sodium hypochlorite promotes invitro smear layer removal and organic matter dissolution during biomechanical preparation. International Endodontic Journal, v. 50, n. 1, p. 106-114, . (13/19789-3, 12/06738-9)
TARTARI, TALITA; BACHMANN, LUCIANO; ALVES MALIZA, AMANDA GARCIA; DE ANDRADE, FLAVIANA BOMBARDA; HUNGARO DUARTE, MARCO ANTONIO; BRAMANTE, CLOVIS MONTEIRO. Tissue dissolution and modifications in dentin composition by different sodium hypochlorite concentrations. Journal of Applied Oral Science, v. 24, n. 3, p. 291-298, . (12/02460-6, 13/19789-3)
TALITA TARTARI; CAROLINE WICHNIESKI; RENATO MENEZES SILVA; ARIADNE LETRA; MARCO ANTONIO HUNGARO DUARTE; CLOVIS MONTEIRO BRAMANTE. Final irrigation protocols can be used to promote stable long-term bond strength of AH Plus to dentin. Journal of Applied Oral Science, v. 31, . (17/22364-5, 18/12690-5, 13/19789-3, 15/09958-8)
TARTARI, T.; WICHNIESKI, C.; BACHMANN, L.; JAFELICCI, JR., M.; SILVA, R. M.; LETRA, A.; VAN DER HOEVEN, R.; DUARTE, M. A. H.; BRAMANTE, C. M.. Effect of the combination of several irrigants on dentine surface properties, adsorption of chlorhexidine and adhesion of microorganisms to dentine. International Endodontic Journal, v. 51, n. 12, p. 1420-1433, . (13/19789-3, 15/09958-8, 12/02460-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TARTARI, Talita. Effects of different irrigation regimes in physico-chemical properties of dentin and consequences of changes in the adhesion of microorganisms and AH Plus sealer. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.

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