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Use of a menthol-based cream in the wound healing

Grant number: 13/22202-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2014
Effective date (End): September 30, 2015
Field of knowledge:Biological Sciences - Pharmacology - Ethnopharmacology
Principal researcher:Claudia Helena Pellizzon
Grantee:Ariane Leite Rozza
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The loss of skin integrity due to physical, chemical or thermal injuries results in the formation of a wound. In consequence, the healing process is triggered, reestablishing the function and integrity of the injured skin. The healing process is classified into three overlapping phases: inflammatory (hemostasis and inflammation), proliferative (granulation tissue formation and reepithelization) and extracellular matrix remodeling phase. Menthol is the main compound of essential oils from genus Mentha, presenting antinociceptive, local anesthetic and antimicrobial effects. This research project aims to evaluate the healing potential from menthol, in a topical cream, in cutaneous wounds, and elucidate the mechanism of action from menthol. Male Wistar rats (n=7) will be distributed into 5 groups: ointment base (control), collagenase cream (positive control), menthol ointment at 2.5, 5 and 10% (w/w). After anesthesia, a circular excision of 2 cm of diameter will be created on the dorsal region of the rats. The wounds will be treated and measured daily for 3, 7 or 21 days (according to the phases described in the literature). After euthanasia, histological (inflammatory cells count, inflammatory score, morphology of collagen fibers), histomorphometrical (regenerative epithelium height measurement) and immunohistochemistry (epidermal and vascular endothelial growth factors, cell proliferation, apoptosis and cytoprotection) analyses will be performed, besides molecular analyses (qPCR and RT-PCR for collagen I and III, epidermal and ²1-transforming growth factor). Analyses of antioxidant activity (glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase and myeloperoxidase) will be performed in the liver of the rats and analyses of anti-inflammatory activity (tumor necrosis factor-±, pro-inflammatory interleukins IL-1² and IL-6 and anti-inflammatory interleukin IL-10) will be performed in the rats' serum. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROZZA, ARIANE LEITE; BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; OLIVEIRA DE SOUZA, EDUARDO; HUSSNI, CARLOS ALBERTO; MARTINEZ, EMANUEL RICARDO MONTEIRO; NOBREGA, RAFAEL HENRIQUE; PELLIZZON, CLAUDIA HELENA. The Use of Menthol in Skin Wound Healing-Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization. PHARMACEUTICS, v. 13, n. 11 NOV 2021. Web of Science Citations: 0.

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