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Comparison of three cryoprotectants in the semen of Mazama americana species collected by artificial vagina

Grant number: 13/22962-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2014
Effective date (End): January 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:José Maurício Barbanti Duarte
Grantee:Maria Clara Lopes Alvarez
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The efficiency of semen cryopreservation is critical for the genomic resource banks construction, which are important tools for maintaining the genetic variability of wild and captive populations, aiming their conservation. One factor that influences the successful of semen cryopreservation is the choice of a cryoprotectant that fits best the characteristics of the ejaculate of a particular species. This study aims to evaluate the efficiency of different penetrating cryoprotectants in the ejaculate of the Mazama americana species. For semen collection, attempts will be made using females in oestrus and the males (n=6) will have the penis deviation for an artificial vagina. After collection, the semen will be divided into three parts, each one will be diluted in a extender containing one of penetrating cryoprotectants to be tested: glycerol (6%), ethylene glycol (3%) and dimethylformamide (5%). To determine which one has the best potential to cryopreserve semen, assessments will be made in pre-freeze and post-thaw samples to determine the vigour, motility, membrane integrity, acrosomal integrity and sperm morphology.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVAREZ, MARIA C. L.; ROLA, LUCIANA D.; DUARTE, JOSE M. B.. Comparison Between Three Cryoprotectants in the Freezing ofMazama americanaSemen Collected by Artificial Vagina. BIOPRESERVATION AND BIOBANKING, . (13/22962-9)

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