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Characterization of adaptative mutations in Escherichia coli

Grant number: 13/24755-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2014
Effective date (End): December 31, 2014
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal researcher:Beny Spira
Grantee:Henrique Iglesias Neves
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In this project, will be selected mutations in the pst operon that result in loss of the capacity to repress the transcription of the PHO-regulon, but maintain the ability to transport Pi. Deletions on Pi transporter will be constructed and the resulting strain will be used to select PHO-constitutive mutants. The locus of the mutation in these mutants will be defined by complementation tests and the affected gene will be sequenced by the Sanger method.

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