Advanced search
Start date

Degradation of black liquor by basidiomycetes fungi from native forest

Grant number: 13/15747-4
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2014
Effective date (End): August 31, 2015
Field of knowledge:Biological Sciences - Ecology - Applied Ecology
Principal Investigator:Regina Teresa Rosim Monteiro
Grantee:Djanira Rodrigues Negrão
Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


This project started at February 2012, collecting 24 isolates of fungi from native forest with the main aim to evaluate their potential for degradation and detoxification of black liquor. This waste, from pulp and paper industry, has environmental importance because its recalcitrant characteristics, like high BOD, QOD, lignin contents, phenolic compounds and pH 14. All fungi were screened according to its enzymatic activity by its growth and decolorization of vinasse and black liquor-agar solid medium (20 and 5%, respectively). In this study, the fungi growth will be verified in black liquor, starting from 10% of concentration, and its detoxification and degradation will be done by the methodology developed in the University of Toronto, Canada, during an internship from August 2012 to February 2013. In this internship we also looked for a detoxification of the black liquor and recovery of lignin. The mycelial growth and lignolytic enzymes activity, like laccases and peroxidases, will be analyzed during fungi growth in liquid state with agitation at 27 ºC, during 18 days. On each three days, supernatant samples will be taken and physicochemical analyses will be done (BOD, COD, metals, loss of color and pH), as well as biomass. In the same experiment, the lignin present in the degraded black liquor will be recovered by acid precipitation and together of its compounds, derived from enzymatic degradation, will be analyzed by size exclusion and gas chromatographic methods, respectively. Losses of toxicity of the black liquor enzymatically modified will be evaluated exposing aquatic organisms standardized in ecotoxicological assays, using Hydra attenuata and Daphnia magma. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NEGRAO, DJANIRA RODRIGUES; MISCHAN, MARTHA MARIA; DE PINHO, SHEILA ZAMBELLO; DE CARVALHO, LIDIA RAQUEL; GOMES, RAFAELA LANCAS; PASSOS, JOSE RAIMUNDO DE SOUZA. How temperature variation affects white-rot fungi mycelial growth dynamics: a nonlinear mixed models approach. FUNGAL BIOLOGY, v. 125, n. 11, p. 860-868, . (13/15747-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
NEGRÃO, Djanira Rodrigues. Modification of lignin structure caused by fungi. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB) Piracicaba.

Please report errors in scientific publications list by writing to: