Advanced search
Start date

Use of PHBV (poli-(3-hidroxibutirato-co-3hidroxivalerato) nanoparticules coupled to busulfan in the sterilization of chicken receptors of germ cell transplantations

Grant number: 13/21829-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2013
Effective date (End): November 30, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Ricardo Jose Garcia Pereira
Grantee:Allison Jun Taguchi Kawaoku
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Conservation of avian germplasm through germ cell transplantation among different individuals or species has been explored as an alternative to circumvent limitations of the available reproductive biotechnologies for this class. In this regard, an essential step in applying such technique is the preparation of the bird recipient, which advocates depletion of endogenous germ cells prior to transplantation. Since there are no mutant birds with impaired spermatogenesis that could suit as germ cell recipients, as in mice, different surgical, physical and chemical methods have been investigated for the generation of avian recipients. However, all procedures tested so far exhibited technical barriers such as partial depletion of germ cells, induction of mutations and high embryonic mortality. In this context, injections of busulfan in ovo are frequently cited by researchers, although it is not effective to completely sterilize recipients. Thus, the present study aims to evaluate if the use of PHBV copolymer nanoparticles coupled with busulfan will provide a greater degree of sterility in one-day-old-chick recipients than the traditional emulsion of busulfan (busulfan/ dimethylformamide/busulfan). The data obtained in this study may contribute to the development of more efficient protocols in the preparation of chicken recipients for the transplantion of germ cells, turning this approach more feasible for both preservation of genetic material and for generation of transgenic birds.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Please report errors in scientific publications list by writing to: