Influence of polymorphisms TaqI and BsmI of vitamin D receptor on recuperation of burned patients

Abstract

Vitamin D is a fat-soluble compound which exerts its biological effects by binding to the vitamin D receptor (VDR). The VDR gene shows polymorphisms in the population, which may present variations in the efficiency of vitamin D actions. Some recent studies have shown nutritional therapy increasing the efficiency of healing process, as occur in burned patients, decreasing the comorbidities involved in this clinical picture. Vitamin D is one of the components present in such therapy, and it is known that efficiency of nuclear VDR activity is modulated by the presence of BsmI and TaqI polymorphisms, establishing a connection between such polymorphisms and response to burns. Therefore our goal will be to detect the presence of BsmI and TaqI polymorphisms of the VDR gene in patients who have suffered burns and compare with the period of hospitalization, development of infection and mortality. Patients admitted to the "Unidade de Queimados" of "Hospital Estadual de Bauru" will be monitored during their recovery and will be recorded hospitalization time, the presence of infection and mortality. In admission are collected 5 ml blood for DNA extraction and will be performed PCR-genotyping, which reveal forms of BsmI and TaqI polymorphisms in the VDR gene in each patient. To evaluate association of polymorphisms with hospitalization time we will use the linear regression analysis, and the association of polymorphisms with the development of infection or mortality will used the logistic regression analysis, always with 5 % of significance level. Polymorphisms related to these aspects of burned patients allow us to infer if the presence of a particular variant can alert to a different behavior during the patient recovery.