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Development, validation and application of clean analytical method for the determination of residues in chicken eggs sulfoquinoxaline

Grant number: 13/18892-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2013
Effective date (End): July 31, 2014
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Eny Maria Vieira
Grantee:Jeferson Fernando Vicentin
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil


The use of veterinary drugs is of great importance in animal production for treatment and prevention of various diseases as well as for development of improved animals, providing improvements in the quality of food produced. However, the application these drugs can result in the presence of residues in food, affecting the health of consumers. Among these drugs, there are the sulfonamides, which belong to a class antibiotic used in both animal and human treatment, and may be present as several residues in animal foods, such as chicken egg which is widely consumed by the population. If these residues are in concentrations above the limit maximum residue levels established by the organs of control, may cause undesirable reactions, as bacterial resistance, poisoning and allergic reactions, compromising security feed the population and making essential control. Given the above, the present project aims to develop, validate and apply a clean analytical method in determining sulphaquinoxaline of sulfonamide residues in chicken eggs, using the stage of preparation Sample technique dispersive liquid-liquid microextraction (DLLME), and analyzes properly said to be made by high performance liquid chromatography with diode array detection(HPLC-DAD).

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