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Synergistic effects between Nosema sp. and insecticide imidacloprid on honeybees africanized Apis mellifera

Grant number: 13/09555-5
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2013
Effective date (End): June 30, 2017
Field of knowledge:Biological Sciences - Zoology - Applied Zoology
Principal Investigator:Osmar Malaspina
Grantee:Hellen Maria Soares Lima
Host Institution: Centro de Estudos de Insetos Sociais (CEIS). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated research grant:12/50197-2 - Action of products used in cultivation of sugar cane on non-target organisms, AP.BIOEN.TEM


The decline of Apis mellifera colonies has become a concern worldwide and this phenomenon, known as colony colapse disorder (CCD), has been attributed to infection of the colonies by Nosema ceranae and to action of the insecticides. Nosema apis and N. ceranae are microsporidian parasite mainly found in the midgut of infected bees. It was demonstrated that the interaction between N. apis, N. ceranae and imidacloprid increased the mortality rates of the honeybees and weakened the colonies. The insecticide imidacloprid acts agonistically on nicotinic acetylcholine receptors of insects and in sublethal doses, this compound impairs learning performance and memory formation, result of morphophysiological changes caused by this insecticide. This work aims to evaluate the synergistic effects between N. ceranae and imidacloprid on mushroom bodies of the nervous system, target structure of this insecticide, and midgut, reservoir of the microsporidian spores and region involved with the metabolization of the insecticides when these are ingested. So, worker honeybees of africanized A. mellifera will be exposed to the microsporidian N. ceranae and/or will be treated orally with imidacloprid. After that, the bees will be dissected and the tissues will be analyzed with light microscopy and confocal microscopy.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GRELLA, TATIANE CAROLINE; SOARES-LIMA, HELLEN MARIA; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO. Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach. Chemosphere, v. 236, . (14/12488-0, 13/09555-5, 17/21097-3)
AZEVEDO, PATRICIA; BUTOLO, NICOLE PAVAN; DE ALENCAR, LUCIANO DELMONDES; SOARES-LIMA, HELLEN MARIA; SALES, VICTOR RIBEIRO; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO. Standardization of in vitro nervous tissue culture for honeybee: A high specificity toxicological approach. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 189, . (14/12488-0, 13/09555-5, 17/21097-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LIMA, Hellen Maria Soares. Combined effects of Nosema ceranae and imidacloprid insecticide on africanized honeybees Apis mellifera. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp).

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