Advanced search
Start date
Betweenand

Biophysical and structural studies of extracellular hemoglobin of Glossoscolex paulistus, and its monomer d and dodecamer (abcd)3 subunits.

Grant number: 13/09829-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2014
Effective date (End): May 30, 2016
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal researcher:Marcel Tabak
Grantee:Francisco Adriano de Oliveira Carvalho
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

Glossoscolex paulistus hemoglobin (HbGp) has a molecular mass of 3.6 MDa, determined by analytical ultracentrifugation (AUC). HbGp presents high oligomeric stability, resistance to oxidation and affinity to oxygen binding. The quaternary structure of this macromolecule consists of 144 globin chains, and 36 additional chains, lacking the heme group, named linkers, organized in a double-layered hexagonal structure. This class of proteins presents a high potential in biomedical applications as a blood substitute. Thus, the annelid hemoglobins are studied by several research groups in the world. AUC studies have shown that HbGp dissociates, under several conditions, such as, alkaline pH, presence of surfactants and urea, into smaller subunits, the dodecamer, the tetramer, the trimer and the monomer d. Recent studies have shown that the HbGp-surfactant interactions induce various processes, such as, protein aggregation, oligomeric dissociation and denaturation. On the other hand, the oligomeric stability is strongly associated to the iron oxidation state, and the specific ligand coordinated to the metal center. Thus, the focus of this post-doctoral project is to characterize the kinetic and thermodynamic processes of HbGp oligomeric dissociation and unfolding, in the presence of urea and guanidine hydrochloride. Additionally, the studies of thermal unfolding, in the presence of small concentrations of both denaturants, will be developed by several spectroscopic and hydrodynamic techniques. These studies will allow evaluating the dissociation mechanism, the stoichiometry of the subunits in the whole oligomeric structure, and the stability of the native protein under different conditions. The comparative study on the effect of two chaotropic agents with different physico-chemical characteristics, a neutral (urea) and a cationic (guanidine hydrochloride) ones, will enable the evaluation of the contribution of the electrostatic interaction in protein-denaturant system. Moreover, studies to characterize the main sites of protein-surfactant interactions in the monomer d, will be performed through crystallization and molecular modeling techniques of this subunit. The molecular modeling studies with the tetramer abcd, trimer abc, monomer d, and dodecamer (abcd)3, will be performed to characterize the interactions between the surfactants and these subunits, aiming to advance in the understanding of the interactions between these subunits in the HbGp oligomeric structure. These studies will be developed using crystallization and molecular modeling tools, as well as, spectroscopic and hydrodynamic techniques, such as, optical absorption, circular dichroism, dynamic light scattering (DLS), electron paramagnetic resonance (EPR) and AUC.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARROS, ANA E. B.; CARVALHO, FRANCISCO A. O.; ALVES, FERNANDA R.; CARVALHO, JOSE W. P.; TABAK, MARCEL. Denaturant effects on HbGp hemoglobin as monitored by 8-anilino-1-naphtalene-sulfonic acid (ANS) probe. International Journal of Biological Macromolecules, v. 74, p. 327-336, . (13/09829-8, 11/09863-6)
CARUSO, CELIA S.; BIAZIN, EZER; CARVALHO, FRANCISCO A. O.; TABAK, MARCEL; BACHEGA, JOSE F. R.. Metals content of Glossoscolex paulistus extracellular hemoglobin: Its peroxidase activity and the importance of these ions in the protein stability. Journal of Inorganic Biochemistry, v. 161, p. 63-72, . (13/09829-8)
ALVES, FERNANDA ROSA; CARVALHO, FRANCISCO ADRIANO O.; CARVALHO, JOSE WILSON P.; TABAK, MARCEL. Interaction of Cationic Dodecyl-trimethyl-Ammonium Bromide with Oxy-HbGp by Isothermal Titration and Differential Scanning Calorimetric Studies: Effect of Proximity of Isoelectric Point. Biopolymers, v. 105, n. 4, p. 199-211, . (13/09829-8)
CARVALHO, FRANCISCO A. O.; ALVES, FERNANDA R.; TABAK, MARCEL. Ionic surfactants-Glossoscolex paulistus hemoglobin interactions: Characterization of species in the solution. International Journal of Biological Macromolecules, v. 92, p. 670-681, . (13/09829-8)
CARVALHO, FRANCISCO A. O.; ALVES, FERNANDA R.; CARVALHO, JOSE W. P.; TABAK, MARCEL. Guanidine hydrochloride and urea effects upon thermal stability of Glossoscolex paulistus hemoglobin (HbGp). International Journal of Biological Macromolecules, v. 74, p. 18-28, . (13/09829-8, 11/09863-6, 13/09349-6)
ALVES, FERNANDA ROSA; CARVALHO, FRANCISCO ADRIANO O.; CARVALHO, JOSE WILSON P.; TABAK, MARCEL. Glossoscolex paulistus Extracellular Hemoglobin (HbGp) Oligomeric Dissociation upon Interaction with Sodium Dodecyl Sulfate: Isothermal Titration Calorimetry (ITC). Biopolymers, v. 101, n. 10, p. 1065-1076, . (13/09829-8, 11/09863-6, 13/09349-6)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.