Influence of platelet-rich plasma, low-level laser therapy or their combination on the healing of periodontal fenestration defects in rats: a histochemical and immunohistochemical study

Abstract

The purpose of this study is to histochemically and immunohistochemically analyze the influence of Platelet-Rich Plasma (PRP), Low-Level Laser Therapy (LLLT) or their combination on the healing of periodontal fenestration defects (PFD) in rats. PDF (3 x 2 x 1 mm) will be surgically created in the mandible of 80 rats. The animals will be randomly divided into 4 groups: Group C (control) - the defect will be filled by blood clot only; Group PRP - the defect will be filled with PRP; Group LLLT - the defect will receive LLLT, will be filled with blood clot and then will be irradiated again; Group PRP/LLLT - the defect will receive LLLT, will be filled with PRP and then will be irradiated again. Animals will be euthanized at either 10 or 30 days postoperative. The area of the original surgical defect and the surrounding tissues will be removed en bloc. Transverse serial sections, 5 µm in thickness, will be obtained in an apico-coronal direction and stained with picrosirius red for histologic analysis or subjected to the indirect immunoperoxidase method for detection of runt-related transcription factor 2 (Runx2), ostepontin (OPN), osteocalcin (OC) and alkaline phosphatase (ALP). The results will be analyzed by polarized light microscopy and light microscopy for histochemical and immunohistochemical analyses, respectively. The data will be submitted to statistical analysis.Key words: Platelet-rich plasma; lasers; regeneration; collagen; immunohistochemistry. (AU)