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Effect of laser phototherapy on the transplantation of a BMP4-loaded hydrogel plus stem cells for dentin-pulp complex regeneration in vivo

Grant number: 13/17268-6
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 02, 2014
Effective date (End): January 01, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Marcia Martins Marques
Grantee:Ivana Marcia Alves Diniz
Supervisor: Songtao Shi
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: University of Southern California (USC), United States  
Associated to the scholarship:12/16552-0 - Effects of low level laser therapy associated to a BMP4 incorporated scaffold on dental pulp stem-cells differentiation and migration. An in vitro study., BP.DR


Cell therapies have been showing great potential for dentin-pulp complex regeneration. Nonetheless, one of the most important concerns related to dentin-pulp complex regeneration is the lack of appropriate blood supply for new tissue morphogenesis. Despite the limited remodeling potential of the dental pulp, especially in mature teeth, this tissue does in fact demonstrate a reparative capacity. Thus, conservative treatments of dental pulp could provide nutritional support to stem cells during implantation of scaffolds. Additionally, remnants of dental pulp are sources of stem cells, which could also be involved in regeneration and remodeling in vivo (cell homing). Moreover, recently, laser phototherapy (LPT) was introduced in the tissue engineering field with promising results. LPT could be an additional component for tissue engineering by modulating cellular functions needed for tissue reconstruction. Applying LPT during scaffold transplantation could increase cell viability, migration and proliferation in and/or towards the biomaterial of stem cells both transplanted in the scaffold and present in the host itself. With this in mind, the objective of this study is to evaluate whether human dental pulp stem cells (hDPSCs) seeded onto a hydrogel with a loaded growth factor (PL-BMP4) can collaborate to regenerate functional pulp and dentin tissues in partially amputated dental pulp. Isolated and characterized hDPSCs will be transduced using a GFP retroviral vector, and then seeded onto a PL-BMP4 scaffold. Molars of Wistar rats will be extracted, opened and pulp tissue partially removed. Then, PL-BMP4 + hDPSCs GFP+ complex will be transplanted into these rat molars empty pulp chambers. Subsequently, rat molars will be sealed with MTA and implanted into renal capsules of immunocompromised mice. Two to three months after transplantation, the mice will be euthanized and their kidneys with the transplanted teeth will be removed for analysis. The formation of a new dental tissue and its characteristics will be assessed by histological, immunohistochemical and immunofluorescent techniques. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DINIZ, IVANA M. A.; CHEN, CHIDER; XU, XINGTIAN; ANSARI, SAHAR; ZADEH, HOMAYOUN H.; MARQUES, MARCIA M.; SHI, SONGTAO; MOSHAVERINIA, ALIREZA. Pluronic F-127 hydrogel as a promising scaffold for encapsulation of dental-derived mesenchymal stem cells. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 26, n. 3, . (13/17268-6)
DINIZ, IVANA M. A.; CARREIRA, ANA C. O.; SIPERT, CARLA R.; UEHARA, CINDI M.; MOREIRA, MARIA S. N.; FREIRE, LAILA; PELISSARI, CIBELE; KOSSUGUE, PATRICIA M.; DE ARAUJO, DANIELE R.; SOGAYAR, MARI C.; et al. Photobiomodulation of mesenchymal stem cells encapsulated in an injectable rhBMP4-loaded hydrogel directs hard tissue bioengineering. Journal of Cellular Physiology, v. 233, n. 6, p. 4907-4918, . (12/16552-0, 13/17268-6)

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