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Effect of an experimental paste with hydroxyapatite nanoparticle and fluoride on bovine enamel demineralization and remineralization in situ

Grant number: 13/03942-7
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2013
Effective date (End): May 31, 2015
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal researcher:Ana Carolina Magalhães
Grantee:Beatriz Martines de Souza
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


This study will evaluate the potential of an experimental bioactive paste based on innovative technology of hydroxyapatite nanoparticles on the reduction of enamel demineralization and the increase of enamel remineralization in situ. Fifteen healthy and adult subjects will take part in this crossover and double-blind study performed in 4 phases of 14 days each (washout: 7 days). Each 2 sound and 2 pre-demineralized bovine enamel specimens will be worn intraorally at each phase. The phases are corresponding to the following treatments: Nanop plus (10% calcium phosphate as HA + 900 ppm F), MI Paste Plus (CPP-ACP + 900 ppm F), F (900 ppm F) and placebo (paste without active ingredients). For that, 240 bovine enamel specimens (4x4 mm) will be selected by using initial surface microhardness (SH initial); half (n=120) will be subjected to demineralization (WS, demineralizing solution with pH 5, for 6 days) and the other half will remain sound (S). The surface microhardness will be measured after producing the lesion (SH lesion). During the preparation of the intraoral appliances, S samples will be protected from disturbance by using plastic mesh to allow biofilm accumulation; while on WS no biofilm accumulation will be induced for allowing remineralization. The initially S specimens will be exposed to severe cariogenic challenge (20% sucrose, 8x5min/day, extraorally, 1 drop/specimen); while the pre-demineralized specimens (WS) will not. The subjects will treat all specimens by applying 1 drop of the solutions (1 paste: 3 water), for 4 minutes, extraorally, 2 times/day (morning and evening). The enamel alterations will be quantified by transversal microradiography (mineral loss- ”Z; lesion depth- µm; the maximum mineral content of surface layer- Zmax; mineral loss over the depth- R values). The data will be subjected to the statistical analysis to compare the treatments (p<0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUZA, BEATRIZ M.; COMAR, LIVIA P.; VERTUAN, MARIELE; NETO, CONSTANTINO FERNANDES; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ. Caries Research, v. 49, n. 5, p. 499-507, . (13/03942-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Beatriz Martines de. Effect of an experimental paste with hydroxyapatite nanoparticles and fluoride on dental demineralization and remineralization in situ. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.

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