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Effect of addition of fluorescein/rhodamine B into non-simplified dentin bonding agents: photophysical and physicochemical approaches

Grant number: 13/13161-2
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2013
Effective date (End): April 08, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Maria Teresa Atta
Grantee:Odair Bim Júnior
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):14/18419-0 - Effect of organic solvent on ternary phase diagram of model dentin adhesive, BE.EP.DR


Adhesive systems have been labeled with fluorescein (FL) and rhodamine B (RB) for the assessment of bonded interfaces via confocal laser scanning microscopy (CLSM). However, setting an optimal concentration of these fluorescent markers depends on both the adhesive photophysical aspects involved in the formation of images in CLSM, and the need to preserve the physicochemical properties of the adhesive systems. This study aims to (i) estimate the lowest serviceable range of concentrations of FL/RB into adhesives for CLSM, and (ii) evaluate the effect of different concentrations of FL/RB on the degree of conversion and wettability of the adhesives. The adhesive systems Adper Scotchbond Multi-Purpose (MP) and Clearfil SE Bond (SE) will be labeled with FL/RB by means of small aliquots of ethanol-FL or ethanol-RB, allowing the use of contractions greatly reduced. Disks of cured adhesives containing FL/RB at different concentrations (mg/mL) will be prepared: 0.50 / 0.10 / 0.02 / 0.01. The fluorescent behavior of the disks will be analyzed by both photoluminescence spectroscopy and CLSM, for assessing physicochemical data (spectroscopy) and visual data (microscopic). The degree of conversion in pure adhesives or labeled ones will be evaluated by Fourier transform infrared spectroscopy. The wettability of the resin in original composition and the modified version will be estimated by contact angle measurements (ADSA method). It is expected that the association of visual, qualitative and quantitative methods offers the awareness for the use of labeled-adhesives in a consistent and well-founded way.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BIM JUNIOR, ODAIR; CEBIM, MARCO A.; ATTA, MARIA T.; MACHADO, CAMILA M.; FRANCISCONI-DOS-RIOS, LUCIANA F.; WANG, LINDA. Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface. Microscopy and Microanalysis, v. 23, n. 1, p. 122-130, . (12/13160-3, 11/14971-2, 13/13161-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BIM JÚNIOR, Odair. Effect of addition of rhodamine B and fluorescein to conventional etch-and-rinse and self-etching adhesive systems: photophysical and physical-chemical aspects. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.

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