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Effects of seminal plasma on motility characteristics and fertility of cryopreserved boar sperm

Grant number: 13/08070-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2013
Effective date (End): December 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:André Furugen Cesar de Andrade
Grantee:Mariana Andrade Torres
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):14/22972-7 - Novel flow cytometer techinique for sperm analysis: validation and practical application, BE.EP.MS


This project aims to verify the modifications generated by the technique of cryopreservation on motility characteristics, and determine if addition/maintenance of boar seminal plasma before or after thawing reduces these effects and improves fertility. For this, ejaculates from six boars will be collected. After analyses, the semen in natura, will be diluted before centrifugation. After this, semen pellet will be divided in two treatments: Control (CON) - suspended with extender for cryopreservation and (PAC) - suspended with extender for cryopreservation within 10 % (v/v) of seminal plasma. Extended semen will be loaded in 0.5 mL straws and then subjected to cryopreservation. Ten straws from each batch will be thawed from group PAC and CON. Forty five mL of Prolimax® extender will be added to CON and PAC groups in order to obtain fifty mL of insemination dose within 60x106sptz/mL. Another ten straws from CON will be thawed and extended with Prolimax®, within 10 % (v/v) of seminal plasma giving rise to the third treatment, group PDC. After addition of treatment the samples remain in the water bath at 37° C until the end of analysis evaluated in periods 0 and 120 minutes. The semen analysis will consist in the computerized analysis of the characteristics of motility (CASA). Fertility test will be performed in seventy five gilts. Inseminations will be performed with three treatments CON (n=25), PAC (n=25) e PDC (n=25). Continuous variables are compared by Tukey test and the fertility and birth rate will be analyzed by Chi-Square non-parametric test, both for P<0.05.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TORRES, M. A.; RAVAGNANI, G. M.; LEAL, D. F.; MARTINS, S. M. M. K.; MURO, B. B. D.; MEIRELLES, F. V.; PAPA, F. O.; DELL'AQUA JUNIOR, J. A.; ALVARENGA, M. A.; MORETTI, A. S.; et al. Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing. JOURNAL OF ANIMAL SCIENCE, v. 94, n. 5, p. 1906-1912, . (13/08070-8, 11/23484-8)
TORRES, MARIANA ANDRADE; DIAZ, ROMMY; BOGUEN, RODRIGO; MASSAMI KITAMURA MARTINS, SIMONE MARIA; RAVAGNANI, GISELE MOURO; LEAL, DIEGO FEITOSA; OLIVEIRA, MELISSA DE LIMA; DONATELLI MURO, BRUNO BRACCO; PARRA, BEATRIZ MARTINS; MEIRELLES, FLAVIO VIEIRA; et al. Novel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?. PLoS One, v. 11, n. 8, . (13/08070-8, 14/22972-7, 11/23484-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TORRES, Mariana Andrade. Effect of seminal plasma on motility characteristics and fertility of cryopreserved boar spermatozoa. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) Pirassununga.

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